@thesis{thesis,
		author={ M. RIZKY FADHILAH   1717011060},
		title ={

 
 KARAKTERISASI KITOSAN DARI FUNGI 19A15-RF (Aspergillus
ochraceus) YANG BERASOSIASI DENGAN SPONS 

},
		year={2022},
		url={http://digilib.unila.ac.id/65606/},
		abstract={
 
Fungi diketahui mengandung kitosan pada dinding selnya. Dalam penelitian ini, fungi
yang digunakan merupakan koleksi dari UPT LTSIT dengan kode 19A15-RF.
Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi kitosan dari fungi
19A15-RF. Fungi diremajakan menggunakan media malt ekstrak agar. Berdasarkan
analisis Filogenetik, isolat 19A15-RF memiliki kemiripan 99,32%  dengan
Aspergillus ochraceus . Fungi dikultivasi skala besar pada media ekstrak malt dengan
waktu variasi 10, 14, dan 18 hari. Pemilihan variasi hari bertujuan untuk melihat
pertumbuhan optimum fungi, mengetahui kemampuan optimum fungi dalam
memproduksi kitosan dan mengetahui derajat deasetilasi optimum. Kitosan diekstrak
dari fungi yang dikultur dalam media malt ekstrak secara Submerged Fermentation
(SmF). Fungi diekstraksi dengan NaOH dan CH
COOH. Kitosan yang diperoleh
dengan masa kultivasi 10, 14, dan 18 hari diperoleh sebanyak 0,274 g, 0,499 g, dan
0,319 g secara berurutan, dan diperoleh persen rendemen kitosan dengan masa
kultivasi 10, 14, dan 18 hari secara berurutan diperoleh sebanyak 0,7%, 1,1%, dan
0,7%. Berdasarkan analisis spektroskopi FTIR, diperoleh gugus fungsi kitosan
meliputi serapan pada bilangan gelombang karbonil, C=O-NHR dapat diamati pada
1640 cm
-1
 dan pita amina, NH
2
 pada 1543-1565 cm
3
-1
. Derajat deasetilasi kitosan yang
dihasilkan pada variasi hari ke 10, 14 dan 18 berturut-turut yaitu 65,85%, 65,15% dan
76,1%. Berdasarkan analisis unsur menggunakan elemental analyzer, jumlah nitrogen
pada kitosan hari ke 10, 14 dan 18 diperoleh berturut turut sebesar 0,78%, 1,26%, dan
0,37%. Informasi awal ini penting, karena dapat digunakan sebagai dasar untuk
pengembangan lebih lanjut dalam pencarian kitosan yang berasal dari fungi asosiasi
spons laut.


Kata kunci : Aspergillus ochraceus, Fungi, Karakterisasi, Kitosan, Spons 
  


 
Fungi are known to contain chitosan in their cell walls. In this study, the fungi used
were collections from UPT LTSIT with code 19A15-RF. This study aimed to isolate
and characterize chitosan from 19A15-RF fungi. Fungi were rejuvenated using malt
agar extract media. Based on phylogenetic analysis, isolate 19A15-RF was 99.32%
similar to Aspergillus ochraceus . Fungi were cultivated on a large scale on malt
extract media with variations of 10, 14, and 18 days. The choice of day variation aims
to see the optimum growth of fungi, to determine the optimum ability of fungi to
produce chitosan and to determine the optimum degree of deacetylation. Chitosan
was extracted from fungi cultured in malt extract media by Submerged Fermentation
(SmF). Fungi were extracted with NaOH and CH
COOH. Chitosan obtained with a
cultivation period of 10, 14, and 18 days was obtained as much as 0.274 g, 0.499 g,
and 0.319 g respectively, and the percentage yield of chitosan with a cultivation
period of 10, 14, and 18 days respectively was 0.7%, 1.1%, and 0.7%. Based on FTIR
spectroscopic analysis, the functional groups of chitosan include absorption at the
carbonyl wave number, C=O-NHR which can be observed at 1640 cm
3
 and the
amine band, NH2 at 1543-1565 cm
-1
. The degree of deacetylation of chitosan
produced on the 10th, 14th and 18th day variations, respectively, was 65.85%,
65.15% and 76.1%. Based on elemental analysis using an elemental analyzer, the
amount of nitrogen in chitosan on days 10, 14 and 18 was obtained at 0.78%, 1.26%,
and 0.37%, respectively. This preliminary information is important, because it can be
used as a basis for further development in the search for chitosan derived from marine
sponge associated fungi.


Keywords: Aspergillus ochraceus, Fungi, Characterization, Chitosan, Sponges.
 
-1}
		}