@thesis{thesis, author={Muhammad Abdul Azis}, title ={PENURUNAN ANGKA KUMAN TELAPAK TANGAN DENGAN HAND SANITIZER NON-ALKOHOL KOMBINASI MINYAK ATSIRI DAUN KEMANGI (Ocimum basillicum) DAN DAUN JERUK NIPIS (Citrus aurantifolia S.)}, year={2022}, url={http://librepo.stikesnas.ac.id/900/}, abstract={Diseases can be transmitted indirectly through food that enters the body such as diarrhea, cholera, dysentery, worms, typhoid, and hepatitis. The lazy habit of washing hands and the incompatibility of hand sanitizers containing chemicals that cause a burning feeling on the skin may trigger the spread of bacteria and viruses from and through the hands to enter the digestive tract by food that is handled and cause disease in humans. Hand sanitizer is an instant health product that can be used to inhibit and kill germs without the need for air, without rinsing, and can be used anytime and anywhere. almost all brands and types of hand sanitizers contain alcohol and triclosan as the main ingredients. Alcohol alone can dry out your hands. Therefore, there is a need for new innovations in the form of non-alcoholic hand sanitizers that are made from natural ingredients and do not cause a burning sensation on the skin. Natural ingredients that can be combined to be developed into non-alcoholic hand sanitizers are basil leaves and lime leaves. The purpose of this study was to determine the decrease in the number of germs on the palms before and after the use of a non-alcoholic hand sanitizer, a combination of essential oils of basil and lime leaves. Testing of hand sanitizers from a combination of natural ingredients in reducing the number of germs on the palms with the pre-test and posttest control methods. The results of the calculation of the decrease in bacterial colonies obtained an average formula 1 of 24.16%, formula 2 of 38.41%, and formula 3 of 43.13%. The data above concludes that the most optimal formula to reduce the number of hand germs is formula 3 with a ratio of active ingredients of essential oils of basil and citrus leaves of 10%: 5%. The factors that cause the number of germs on the hands can be caused by the activities of different respondents, while the factors that affect the size of the decrease in germs can be caused by the high and low antibacterial content of each active substance used.} }