@thesis{thesis,
		author={Sembiring  Helmina Br.},
		title ={Isolasi dan Penentuan Struktur Kimia Senyawa Flavonoida Daun Benalu Jeruk (Scurrula fusca G. Don) Serta Uji Aktivitas Antioksidan},
		year={2018},
		url={},
		abstract={The aims of this research are to isolate, examine antioxidant activity and
chemical structure of flavonoid compounds from citrus parasite leaves (Scurrula fusca
G.Don). Flavonoid compounds isolated from the leaves of citrus parasite by extracting
3000 g leaves powder using methanol. Methanol extract re-extracted with ethyl
acetate. Ethyl acetate extract was partitioned with mixture of methanol and n-hexane.
Methanol layer hydrolyzed with HCl 6% and obtained 3.02 g (0.10%) aglycone of
flavonoids. Aglycone of flavonoids were isolated and purified by column
chromatography. Pure compounds F3 (10 mg) was obtained as a brownish green
amorphous solid and F4 (14 mg) as a yellow amorphous solid. The antioxidant
activity of both compounds were investigaed by DPPH free radical scavenging method
using ascorbic acid as a control positive. Antioxidant activity of F3 and F4 obtained
with IC50 values of 9.383 ?g / ml and 8.713 g? / ml respectively. The antioxidant
activity of both compounds classified as very powerful because they have IC50 values
lower than ascorbic acid antioxidant activity with IC50 value of 12.08 g? / ml.
Determination of the chemical structure of both compounds F3 and F4 done by
interpretation of spectral data of spectroscophy FT-IR, UV, 1D NMR (1H NMR, 13C
NMR, and DEPT),2D NMR (1H-1H1 COSY, 1H-13C HMBC, 1H-13C HMQC) dan LCMS.
Based on these data, F3 expressed as 3.7-dihydroxy-5-methyl-3', 4'-
dihydrofurano-flavonol and F4 as 3,5,7,4'-tetrahydroxyflavonol or Kaempferol.}
		}