@thesis{thesis,
		author={Restuati  Martina},
		title ={Studi Aktifitas Immunostimulan Daun Buas Buas (Premna pubescens. Blumue) pada Tikus Putih (Rattus norvegicus)},
		year={2018},
		url={},
		abstract={Immunostimulant is a substance to increase the immune responsiveness. Premna pubescens.Blumue (buasbuas) has immunostimulant activity by the presence of Apigenin. Apigenin value on Premna pubescens.Blumue was 28.45mcg/ml.This research used 24 Wistar ratsdivided into 4 treatment groups which each A0 was control wich given normal food, A1 was given 250 mg Ethanol Ectract of Premna (EEP)/kg bw, A2 was given 250 mg EEP/kgbw + SRBC 1% and A3 given SRBC only.EEP was given orally for 30 days and SRBC was given on 8th and 15th day of treatment.Blood collection doneon 31th day of treatment. Imunostimulant activity done with measurement of hematology (hemoglobin, hematocrit, erythrocyte and platelet), sellular (leucocyte, lymphocyte, monocyte), humoral(antibody titer, IgM, IgG and Lisozim) and histology (kidney and spleen). For antibody titer and immunoglobulin M and G analyzed by hemaglutination and ELISA technique.Based ANOVA test, leucocyte, lymphocyte and plateletswere have a significant effect. Leucocyte and antibody titer at A2significant increase compare with A3(9.88±2.31>7.52±1.82 x103/mm3 dan 7.17±0.75>6.67±1.51). IgG and IgM have the same result that A2was the highest (3.96±and 9.48±5.90 mg/ml). For Lysozyme measurement show that A2was the highest 0.04?g/ml.Antibody titer and IgM significantly increased compared to the control. For histological overview, centrum arteriole and centrum germinal at A2 is the highest value. The whole result that EEP have immunostimulant activity, because it enhance immune responses in leucocyte, lymphocyte, platelets, antibody and IgM.}
		}