@thesis{thesis,
		author={humaryanto  humaryanto},
		title ={THE REGULATION OF KLF4, CMYC, miR-302b, AURKA, Ascl2,
Mdm2 AND NFKB GENE EXPRESSIONS IN LOW-,
INTERMEDIATE-, HIGH GRADE SOFT TISSUE SARCOMAS
AT FORMALIN FIXED PARAFFIN EMBEDDED (FFPE},
		year={2016},
		url={http://repository.unja.ac.id/12232/},
		abstract={Soft Tissues Sarcomas (STS) account for 1-2% of all malignancies in adults and around
6% in children are a heterogeneous group of tumors with >70 different entities. The
histology gradation of sarcoma is an important indicator to assess the aggressiveness
and the prognosis for the risk of metastasis. The histological grade describes a
molecular morphology translation process that illustrates the progression of tumor
cells. Its mechanism and regulation still unclear. In this study, we investigated
regulation of gene expression of KLF4, cMyc, miR-302b, Aurka, Ascl2, Mdm2 and
NFKB at level of RNA from Formalin Fixed Paraffin Embedded of low grade,
intermediate grade and high grade soft tissue sarcoma. The genes expression was
examined using quantitative Real Time PCR (qRT-PCR) and we analyzed the relative
gene expression using the 2-??Ct method. We identified 23 samples of STS cases in
Jambi city, from 2007 till 2013 consist of 6 cases low grade, 9 cases intermediate grade
and 8 cases high grade. Statistical tests were performed on groups identified by target
genes with soft tissue sarcoma grade, to find discriminating each target genes
expression. There were a significant difference averages of ?Ct KLF4, cMyc and Aurka
expression at low-, intermediate- and high-grade STS (p=0.037/ANNOVA, p=0.025,
p=0.039, both with Kruskall Wallis) and also a significant positive correlation between
KLF4, cMyc, Aurka and Ascl2 expression with a difference grading of STS (p<0.05,
Spearman). A relative quantification by normalized target gene relative to GAPDH as
an internal gene, describes the changes in expression of target genes from high,
intermediate and low grade STS. There is a relative change in mRNA genes expression
of KLF4, c-Myc, Aurka and Ascl2 which is more high the histological grade more
supressed the gene expression, and it is a contrary with miR302b. Our result suggests
that KLF4, cMyc and Aurka gene expression has a potential prognostic/predictive
value for soft tissue sarcoma.
Keyword: soft tissue sarcoma ? grade- Aurka ? cMyc ? Ascl2- KLF4-miR302b-Mdm2-
NFKB}
		}