Uji Daya Hambat Ekstrak Dan Fraksi N-Heksan, Fraksi Etil Asetat, Fraksi Air Daun Kersen (Muntingia calabura L) Terhadap Bakteri Staphylococcus aureus ATCC 25923.
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Institusion
Universitas Duta Bangsa
Author
ANGGRAINI, PONCO HARUM
Subject
RS Pharmacy and materia medica
Datestamp
2022-07-19 02:32:26
Abstract :
ABSTRACT N-HEXAN EXTRACT AND FRACTION INVOLVEMENT TEST, ETHLACETATE FRACTION, KERSEN LEAF WATER FRACTION (Muntingia calabura L) AGAINST BACTERIA Staphylococcus aureus ATCC 25923 Ponco Harum Anggraini Cherry leaves (Muntingia calabura L.) contain many flavonoids, namely flavones, flavanones, and flavans so that they can be antibacterial. This study aims to determine the inhibitory ability of extracts and fractions of N-Hexane, Ethyl Acetate fraction, Kersen Leaf Water fraction (Muntingia calabura L) against Staphylococcus aureus ATCC 25923 and to determine the most active fraction to inhibit bacteria. Extract and fraction tests were carried out using the diffusion and dilution method on the extract sample macerated with 96% ethanol and then continued with the fractionation process. Tests using the diffusion method using a concentration of 1%,5%,10%,15%,20% using a positive control ciprofloxacin 0.5% and the dilution method with concentrations of 50%, 25%, 12.5%, 6.25%, 3.12% , 1.56%, 0.78%, 0.39%, 0.195% and 0.098%. The results of the antibacterial activity test showed that the extract, N- Hexane, Ethyl Acetate, and Water fractions had inhibitory power against bacteria. The most active fraction in this study was the ethyl acetate fraction with the inhibitory power of the ethyl acetate fraction at a concentration of 20% with an average inhibition zone of 11.6 mm. The results of the dilution test of the Ethyl Acetate Fraction with KBM at a concentration of 12.5%.
ABSTRACT N-HEXAN EXTRACT AND FRACTION INVOLVEMENT TEST, ETHLACETATE FRACTION, KERSEN LEAF WATER FRACTION (Muntingia calabura L) AGAINST BACTERIA Staphylococcus aureus ATCC 25923 Ponco Harum Anggraini Cherry leaves (Muntingia calabura L.) contain many flavonoids, namely flavones, flavanones, and flavans so that they can be antibacterial. This study aims to determine the inhibitory ability of extracts and fractions of N-Hexane, Ethyl Acetate fraction, Kersen Leaf Water fraction (Muntingia calabura L) against Staphylococcus aureus ATCC 25923 and to determine the most active fraction to inhibit bacteria. Extract and fraction tests were carried out using the diffusion and dilution method on the extract sample macerated with 96% ethanol and then continued with the fractionation process. Tests using the diffusion method using a concentration of 1%,5%,10%,15%,20% using a positive control ciprofloxacin 0.5% and the dilution method with concentrations of 50%, 25%, 12.5%, 6.25%, 3.12% , 1.56%, 0.78%, 0.39%, 0.195% and 0.098%. The results of the antibacterial activity test showed that the extract, N- Hexane, Ethyl Acetate, and Water fractions had inhibitory power against bacteria. The most active fraction in this study was the ethyl acetate fraction with the inhibitory power of the ethyl acetate fraction at a concentration of 20% with an average inhibition zone of 11.6 mm. The results of the dilution test of the Ethyl Acetate Fraction with KBM at a concentration of 12.5%.
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