UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI N-HEKSAN, FRAKSI ETIL ASETAT, FRAKSI AIR DARI DAUN PEGAGAN (Centella asiatica (L.) Urb) TERHADAP BAKTERI Escherichia coli ATCC 25922 SECARA DIFUSI DAN DILUSI
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Institusion
Universitas Duta Bangsa
Author
SANDY, MONICA
Subject
R Medicine (General)
Datestamp
2022-07-18 07:42:42
Abstract :
ABSTRACT ANTIBACTERIAL ACTIVITY TEST OF THE EXTRACT AND N-HEXAN FRACTION, ETHYL ACETATE FRACTION, WATER FRACTION FROM PEGAGAN LEAVES (Centella asiatica (L.) Urb) AGAINST THE BACTERIA Escherichia Coli ATCC 25922 BY DIFFUSION AND DILUTION Monica Sandy Pegagan leaves (Centella asiatica (L.) Urb) is one of the plants that can be used as an antibacterial. Pegagan leaves contain chemical flavonoids, tannins and saponins. The purpose of this study was to determine the antibacterial activity of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction from pegagan leaves and to determine Minimum Inhibitory Concentration (MIC) and Minimum Kill Concentration (MBC) of the most active fraction of pegagan leaf on the growth of Escherichia coli ATCC 25922. Pegagan leaf powder was macerated using 96% ethanol, then fractionated using n-hexane, ethyl acetate, and water as solvents. The extract, n-hexane fraction, ethyl acetate fraction and water fraction were tested for antibacterial activity using diffusion and dilution methods. Diffusion method with concentration 1%, 5%, 10%, 15%, 20% and dilution method with concentration 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0, 78%, 0.39%, 0.195% and 0.098% against Escherichia coli ATCC 25922. The results of the antibacterial activity test using the diffusion method showed that the extract, n-hexane fraction, ethyl acetate fraction and water fraction of pegagan leaf could inhibit bacterial growth in the presence of inhibitory power. The 20% ethyl acetate fraction was the most effective because it had the highest average inhibition of 13.67 mm. The results of the dilution method test showed that the MBC value of the ethyl acetate fraction was 12.5%. Based on the results of the study, it can be concluded that the ethyl acetate fraction is the most active fraction.
ABSTRACT ANTIBACTERIAL ACTIVITY TEST OF THE EXTRACT AND N-HEXAN FRACTION, ETHYL ACETATE FRACTION, WATER FRACTION FROM PEGAGAN LEAVES (Centella asiatica (L.) Urb) AGAINST THE BACTERIA Escherichia Coli ATCC 25922 BY DIFFUSION AND DILUTION Monica Sandy Pegagan leaves (Centella asiatica (L.) Urb) is one of the plants that can be used as an antibacterial. Pegagan leaves contain chemical flavonoids, tannins and saponins. The purpose of this study was to determine the antibacterial activity of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction from pegagan leaves and to determine Minimum Inhibitory Concentration (MIC) and Minimum Kill Concentration (MBC) of the most active fraction of pegagan leaf on the growth of Escherichia coli ATCC 25922. Pegagan leaf powder was macerated using 96% ethanol, then fractionated using n-hexane, ethyl acetate, and water as solvents. The extract, n-hexane fraction, ethyl acetate fraction and water fraction were tested for antibacterial activity using diffusion and dilution methods. Diffusion method with concentration 1%, 5%, 10%, 15%, 20% and dilution method with concentration 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0, 78%, 0.39%, 0.195% and 0.098% against Escherichia coli ATCC 25922. The results of the antibacterial activity test using the diffusion method showed that the extract, n-hexane fraction, ethyl acetate fraction and water fraction of pegagan leaf could inhibit bacterial growth in the presence of inhibitory power. The 20% ethyl acetate fraction was the most effective because it had the highest average inhibition of 13.67 mm. The results of the dilution method test showed that the MBC value of the ethyl acetate fraction was 12.5%. Based on the results of the study, it can be concluded that the ethyl acetate fraction is the most active fraction.
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