UJI VIRULENSI MIKROBA ENDOFIT INDIGENOUS SEBAGAI UPAYA MEMINIMALISIR INFEKSI SOYBEAN MOSAIC VIRUS (SMV) DAN MENINGKATKAN KETAHANAN INDUKSI TANAMAN KEDELAI (Glycine max (L.) Merril)
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Institusion
Universitas Muhammadiyah Malang
Author
Parlindo, Fitra
Subject
S Agriculture (General)
Datestamp
2018-10-18 02:56:47
Abstract :
Soybean Mosaic Virus (SMV) is a virus that is very detrimental to soybean cultivation. This study aims to determine the types of indigenous endophytic microbes in soybean crop and test their ability to suppress SMV infection, also the effects on induction resistance of soybean crop. This study used an exploration method. The effectiveness test of the application used a simple Randomized Complete Block Design (RCBD). The results of exploration of endophytic fungi amounted to 11 isolates, and 3 isolates for bacteria. So that, each treatment (including positive and negative controls treatment) amounted to 35 treatments for fungi applications, and 11 treatments for bacteria. The statistical analysis used was variance analysis (ANOVA) with a 5% DMRT (Duncan's Multiple Range Test). Endophytic fungi had been isolated from all plant tissues, except pods. The highest diversity of endophytic fungi was found in root and stem tissues, which were 4 isolates respectively. The identities of endophytic fungi that had been identified were Fusarium sp., Verticilllum sp., Alternaria sp., Aspergillus sp., and Penicillium sp. The other 6 isolates couldn’t be identified. Endophytic bacteria were only isolated from pods, roots and soil tissue. All bacteria were Gram-Negative bacteria. The results of Variance analysis (ANOVA) on intensity of mosaic virus attacks on the treatment of endophytic fungi showed a calculated F value > F table 5%, which indicated that all treatments used had a very real effect. Some of the symptoms seen in the leaves of the virus-infected plants in the treatment of endophytic fungi and endophytic bacteria were mosaics and striped, and the color changes of the leaves become stronger or even yellow, the leaf surface becomes uneven, and the leaves looked thicker. More severe changes were leaf malformations that caused the shape of the leaves become contract and dwarf. The treatment of CKB4 (10-1) was the best treatment in suppressing the intensity of virus attacks, with the lowest intensity of virus attacks, 5.02%. Whereas for treatment with bacteria, the treatment of BKA (10-1) was the best treatment with a virus attack intensity of 17.65%.
Soybean Mosaic Virus (SMV) is a virus that is very detrimental to soybean cultivation. This study aims to determine the types of indigenous endophytic microbes in soybean crop and test their ability to suppress SMV infection, also the effects on induction resistance of soybean crop. This study used an exploration method. The effectiveness test of the application used a simple Randomized Complete Block Design (RCBD). The results of exploration of endophytic fungi amounted to 11 isolates, and 3 isolates for bacteria. So that, each treatment (including positive and negative controls treatment) amounted to 35 treatments for fungi applications, and 11 treatments for bacteria. The statistical analysis used was variance analysis (ANOVA) with a 5% DMRT (Duncan's Multiple Range Test). Endophytic fungi had been isolated from all plant tissues, except pods. The highest diversity of endophytic fungi was found in root and stem tissues, which were 4 isolates respectively. The identities of endophytic fungi that had been identified were Fusarium sp., Verticilllum sp., Alternaria sp., Aspergillus sp., and Penicillium sp. The other 6 isolates couldn’t be identified. Endophytic bacteria were only isolated from pods, roots and soil tissue. All bacteria were Gram-Negative bacteria. The results of Variance analysis (ANOVA) on intensity of mosaic virus attacks on the treatment of endophytic fungi showed a calculated F value > F table 5%, which indicated that all treatments used had a very real effect. Some of the symptoms seen in the leaves of the virus-infected plants in the treatment of endophytic fungi and endophytic bacteria were mosaics and striped, and the color changes of the leaves become stronger or even yellow, the leaf surface becomes uneven, and the leaves looked thicker. More severe changes were leaf malformations that caused the shape of the leaves become contract and dwarf. The treatment of CKB4 (10-1) was the best treatment in suppressing the intensity of virus attacks, with the lowest intensity of virus attacks, 5.02%. Whereas for treatment with bacteria, the treatment of BKA (10-1) was the best treatment with a virus attack intensity of 17.65%.
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