Institusion
Universitas Pakuan
Author
Novel, Novel
Heliawati, Leny
Warnasih, Siti
Subject
Kimia
Datestamp
2024-05-17 03:24:30
Abstract :
KTIVITAS ANTIOKSIDAN EKSTRAK DAUN KETAPANG
(Terminalia cattapa L.) DENGAN BERBAGAI METODE DAN UJI
SITOTOKSIKNYA
Novel Suci Nurania)
, Leny Heliawati a), Siti Warnasih a*)
a) Department of Chemistry, Pakuan University, Bogor
*) Corresponding Author: sitiwarnasih@unpak.ac.id
ABSTRACT
Ketapang leaves (Terminalia catappa L.) are wild plants that contain secondary metabolite
compounds, namely flavonoids, triterpenoids, and tannins that have potential as antioxidants.
Flavonoid metabolite compounds in ketapang leaves are thought to have anticancer activity and
reduce cell viability. This study aims to determine the antioxidant and cytotoxic activity of methanol
extracts of ketapang leaves (Terminalia catappa L.) and identify secondary metabolite compounds
using GC-MS, which later can have potential as anticancer. The study began with maceration
extraction using 96% methanol then fractionated with n-Hexan and Ethyl Acetate. Antioxidant
activity was tested using DPPH, CUPRAC and FRAP methods. Furthermore, the best antioxidant
activity value from the three methods was then tested for cytotoxicity using the BSLT (Brine Shrimp
Lethality Test) method and identified active compounds using GC-MS. The results showed that
methanol extract, N-Hexan fraction and Ethyl acetate fraction of ketapang leaves gave the highest
antioxidant activity using DPPH, CUPRAC and FRAP methods with the best results with levels of
18.2855; 302.7199; and 180.4672 µg vitamin C/mg extract in methanol extract. The results of
probit analysis in the cytotoxic test showed that the methanol extract had an LC50 value of 410.96
ppm so that the methanol extract was toxic. There are 23 compounds based on GC-MS tests on
methanol extracts, one of which is epicatechin, Epigallocationchin gallate, and piperidine
compounds which have potential as anticancer.
Keywords: Ketapang Leaf (Terminalia Catappa L), Antioxidant, Cytotoxi, and GC-MS