Abstract :
Kebutuhan akan antibakteri sangat besar sebagai pengobatan penyakit-penyakit infeksi, namun pada kenyataannya didapatkan penggunaan antibakteri yang tidak tepat guna. Tujuan penelitian ini adalah mengetahui potensi air perasan jeruk nipis (Citrus aurantifolia Swingle.) sebagai antibakteri yang mampu menghambat pertumbuhan bakteri Staphylococcus epidermidis. Penelitian ini merupakan penelitian kuantitatif yang menggunakan metode eksperimental dengan design post test only control grup design, Jumlah perlakuan pada penelitian ini adalah 5 perlakuan perasan Jeruk Nipis (Citrus auranitifolia S.) dengan konsentrasi 25%, 50%, 75%, 100%, dan kontrol positif menggunakan kloramfenikol dengan 5 kali ulangan. Analisis data yang digunakan adalah uji statistik one way ANOVA menggunakan program SPSS (Statistical Product of Service Solution) for Windows versi 16.00. Dari hasil penelitian dapat disimpulkan Air perasan jeruk nipis (Citrus aurantifolia Swingle.) memiliki potensi sebagai antibakteri karena dalam pengujian mampu menghambat pertumbuhan bakteri Staphylococcus epidermidis. Konsentrasi air perasan jeruk nipis (Citrus aurantifolia Swingle.) yang mempunyai zona hambat paling besar yang mampu menghambat pertumbuhan Staphylococcus epidermidis adalah pada konsentrasi air perasan jeruk nipis 100% karena memiliki rata-rata 11,48mm. Konsentrasi Hambat Minimum (KHM) dari perasan jeruk nipis (Citrus aurantifolia Swingle.) yang mampu menghambat pertumbuhan Staphylococcus epidermidis adalah pada konsentrasi air perasan jeruk nipis 75% karena memiliki rata-rata 6,28. Konsentrasi Bunuh Minimum (KBM) dari perasan jeruk nipis (Citrus aurantifolia Swingle.) yang mampu membunuh Staphylococcus epidermidis adalah pada Konsentrasi air perasan jeruk nipis 50%.
Kata kunci : antibakteri, air perasan jeruk nipis, staphylococcus epidermidis
Abstract
The need for anti-bacterial is very large as a treatment for infectious diseases, but the act is that the use of anti-bacterial is not appropriate. The purpose of the study was to determine the potential of lime juice (Citrus aurantifolia Swingle) as an anti-bacterial that can inhibit Staphylococcus epidermidis bacterial growth. This research was a quantitative study using experimental methods with post test only control grup design, the number of treatments in this study was 5 treatments of lime juice (Citrus auranitifolia S.) with a concentration of 25%, 50%, 75%, 100%, and the positive control used Chloramphenicol with five replications. The data analysis used statistic test of one way ANOVA with SPSS program (Statistical Product of Service Solution) for Windowsversi 16.00. The results of the study could be concluded that the lime juice (Citrus aurantifolia Swingle) had potential as an anti-bacterial because it was able to inhibit the Staphylococcus epidermidis bacterial growth. The concentration of the lime juice (Citrus aurantifolia Swingle) which had the biggest inhibition zone that was able to inhibit the growth was the concentration of 100 percent due to an average of 11,48 mm. The minimum inhibitory concentration of the lime juice (Citrus aurantifolia Swingle) which could inhibit the bacterial growth was the concentration of 75 percent due to an average 6,28. The minimum kill concentration of the lime juice (Citrus aurantifolia Swingle) which was able to kill the Staphylococcusepidermidis bacteria was at the concentration of 50 percent
Keywords : Anti-Bacterial Activity, Lime Juice, Staphylococcus Epidermidis