UJI DAYA HAMBAT KOMBINASI EKSTRAK ETANOL DAUN
SELEDRI (Apium graveolens L.) DAN DAUN SIRIH (Piper betle L.)
TERHADAP Escherichia coli ATCC 25922
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Kurnia, Putri Anggraini
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2020-12-23 02:50:24
Abstract :
Celery leaves and betel leaves contain chemical compounds flavonoids, saponins and tannins that can inhibit the growth of bacteria Escherichia coli. This study aims to determine the inhibitory combination of ethanol extract of celery leaves and betel leaves against Escherichia coli ATCC 25922 with a diffusion method. The extraction method used is macerated with 70% ethanol. The combination of celery leaf extract concentration and betel leaves are used is 25%: 75%; 50%: 50% and 75%: 25%. Positive control used is the antibiotic cefotaxime 30 µg. Measuring the diameter of inhibition zone of Escherichia coli using the diffusion method of Kirby and Bauer and analyzed statistical methods One Way ANOVA. The results of this study demonstrate that the combination of extracts of celery leaves and betel leaf can inhibit the growth of bacteria Escherichia coli ATCC 25922. The results of the positive control inhibition zone, a combination of 25%: 75%; 50%: 50%; 75%: 25% and negative controls resulted in inhibition zone diameter 39.16 mm; 27.03 mm; 22.66 mm; 21,60 mm and 6.0 mm. The combination of extracts of celery leaves and betel leaves 25%: 75% do not have a potential comparable to the antibiotic cefotaxime 30 g with a significance value of 0.000, which means that there are significant differences in the distribution of the results of inhibition in positive control or comparison combination of extracts. The combination of extracts of celery leaves and betel leaf that produces the highest inhibition zone diameter on a combination of 25%: 75%.
Celery leaves and betel leaves contain chemical compounds flavonoids, saponins and tannins that can inhibit the growth of bacteria Escherichia coli. This study aims to determine the inhibitory combination of ethanol extract of celery leaves and betel leaves against Escherichia coli ATCC 25922 with a diffusion method. The extraction method used is macerated with 70% ethanol. The combination of celery leaf extract concentration and betel leaves are used is 25%: 75%; 50%: 50% and 75%: 25%. Positive control used is the antibiotic cefotaxime 30 µg. Measuring the diameter of inhibition zone of Escherichia coli using the diffusion method of Kirby and Bauer and analyzed statistical methods One Way ANOVA. The results of this study demonstrate that the combination of extracts of celery leaves and betel leaf can inhibit the growth of bacteria Escherichia coli ATCC 25922. The results of the positive control inhibition zone, a combination of 25%: 75%; 50%: 50%; 75%: 25% and negative controls resulted in inhibition zone diameter 39.16 mm; 27.03 mm; 22.66 mm; 21,60 mm and 6.0 mm. The combination of extracts of celery leaves and betel leaves 25%: 75% do not have a potential comparable to the antibiotic cefotaxime 30 g with a significance value of 0.000, which means that there are significant differences in the distribution of the results of inhibition in positive control or comparison combination of extracts. The combination of extracts of celery leaves and betel leaf that produces the highest inhibition zone diameter on a combination of 25%: 75%.
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