UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BUAH KECIPIR
(Psopocarpus tetragonolobus L.) DENGAN METODE DPPH
(1,1-Diphenyl-2-picrylhdrazyl)
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Septi, Nur Handayani
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2020-12-23 02:50:47
Abstract :
Winged bean (Psopocarpus tetragonolobus L.) is a plant widely consumed by the community with abundant active substances. Winged bean contain Flavonoids, phenolic, vitamin C and Vitamin E which can be used as an antioxidant. Antioxidant compounds can be used to capture free radicals. The purpose of this research is to know antioxidant activity of ethanol extract of winged bead fruit and to know at concentration how many extract can reach IC50. Extraction method used was maceration with 70% ethanol solvent. Antioxidant activity was tested using DPPH free radical (1,1-diphenyl-2- picrylhydrazyl). The results showed that inhibition of free radical with concentration average of 100, 150, 200, 250, and 300 ppm was 36,7149%, 42,1111%, 54,0938%, 62,1881%, and 68,6094%. Tests with DPPH method showed moderate antioxidant activity with IC50 value of 183,6786 ppm.
Winged bean (Psopocarpus tetragonolobus L.) is a plant widely consumed by the community with abundant active substances. Winged bean contain Flavonoids, phenolic, vitamin C and Vitamin E which can be used as an antioxidant. Antioxidant compounds can be used to capture free radicals. The purpose of this research is to know antioxidant activity of ethanol extract of winged bead fruit and to know at concentration how many extract can reach IC50. Extraction method used was maceration with 70% ethanol solvent. Antioxidant activity was tested using DPPH free radical (1,1-diphenyl-2- picrylhydrazyl). The results showed that inhibition of free radical with concentration average of 100, 150, 200, 250, and 300 ppm was 36,7149%, 42,1111%, 54,0938%, 62,1881%, and 68,6094%. Tests with DPPH method showed moderate antioxidant activity with IC50 value of 183,6786 ppm.
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