PENETAPAN KADAR FLAVONOID TOTAL DAN
AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAN FRAKSI
DAUN SELEDRI (Apium graveolens L.) DENGAN METODE ABTS
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Indraningsih, Indraningsih
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2020-12-23 06:29:59
Abstract :
Celery leaves as one of the plants that are widely used by people as a food flavoring. Celery leaves can be used as antihypertensive, anti-rheumatic, and reduce uric acid, cause the celery leaves contains flavonoid compounds. This study aims to determine the total flavonoid levels and antioxidant activity of the etanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. In the research, the ethanol extract of celery leaves (Apium graveolens L.) was obtained by maceration using 70% ethanol for 5 days and remaceration for 2 days, and then fractionated by liquid extraction method using separating funnel. The antioxidant activity was determined using ABTS radicals. Ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fractions had total flavonoid that equivalent of quersetin were 63.16%, 83.39%, 88.89%, 67.55% respectively. Antioxidant activity was expressed as IC50 values in the extract, the n-hexane fraction, the ethyl acetate fraction, and water fraction were 24.63?0.23 ppm, 17.35?0.22 ppm, 15.50?0.14 ppm, 17.66?0.28 ppm respectively. Based on the result obtained, the antioxidant potency of extract and fraction were successively of ethyl acetate fraction ? n-hexane fraction ? water fraction ? extract. Statistical analysis using one way ANOVA obtained a significant value of p?0.05. The result indicate there are differences in total flavonoid content and antioxidant activity between ethanol extract and fractions from celery leaf.
Celery leaves as one of the plants that are widely used by people as a food flavoring. Celery leaves can be used as antihypertensive, anti-rheumatic, and reduce uric acid, cause the celery leaves contains flavonoid compounds. This study aims to determine the total flavonoid levels and antioxidant activity of the etanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. In the research, the ethanol extract of celery leaves (Apium graveolens L.) was obtained by maceration using 70% ethanol for 5 days and remaceration for 2 days, and then fractionated by liquid extraction method using separating funnel. The antioxidant activity was determined using ABTS radicals. Ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fractions had total flavonoid that equivalent of quersetin were 63.16%, 83.39%, 88.89%, 67.55% respectively. Antioxidant activity was expressed as IC50 values in the extract, the n-hexane fraction, the ethyl acetate fraction, and water fraction were 24.63?0.23 ppm, 17.35?0.22 ppm, 15.50?0.14 ppm, 17.66?0.28 ppm respectively. Based on the result obtained, the antioxidant potency of extract and fraction were successively of ethyl acetate fraction ? n-hexane fraction ? water fraction ? extract. Statistical analysis using one way ANOVA obtained a significant value of p?0.05. The result indicate there are differences in total flavonoid content and antioxidant activity between ethanol extract and fractions from celery leaf.
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