UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI DARI
BONGGOL PISANG KEPOK (Musa balbisiana Colla.) TERHADAP
Escherchia coli ESBL (Extended Spectrum Beta Lactamase)
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Saras, Oktavianty Ayu Puspita
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2020-12-23 06:30:08
Abstract :
Kepok banana weevils contain flavonoid compounds that can be used as antibacterial agents. This study aims to prove the ethanol extract and fractions from the ethanol extract of kepok banana hump in inhibiting Escherchia coli ESBL (Extended Spectrum Beta Lactamase) bacteria and comparing its inhibitory ability with Chloramphenicol positive control. Kepok banana weevils were macerated with 96% ethanol then dissolved in warm water and fractionated using n-hexane and ethyl acetate solvents. Antibacterial testing was carried out on the extract, n-hexane fraction, ethyl acetate fraction and water fraction of Kepok banana hump. The method used for testing antibacterial activity is the diffusion method. The negative control used DMSO 10%, while the positive control used was chloramphenicol. The results are shown from the radical inhibition zone diameter. Antibacterial statistical analysis used one way ANOVA. The radical inhibition zone of ethanol extract with a concentration of 25% was 6.66 mm, a concentration of 50% was 7 mm, while for a concentration of 100% was 6 mm. The n-hexane fraction at concentrations of 25%, 50% and 100% gave the same radical zone, namely 6 mm. Ethyl acetate fraction with 25% concentration of radical inhibition zone obtained was 16.95 mm, 50% concentration was 13.3 mm while at 100% concentration was 11.16 mm. The concentration of water fraction 25% and 50% obtained 6 mm radical zone, 100% concentration obtained a radical inhibition zone of 6.33 mm. Ethyl acetate fraction with 25% concentration gave a bigger radical inhibition zone against Escherichia coli ESBL, which was 16.95 mm including intermediates, not yet equivalent to the positive control chloramphenicol which had a radical inhibition zone of 31.9 mm (sensitive). The data also supported statistically there was a significant difference (p <0.05) between the ethyl acetate and chloramphenicol fractions.
Kepok banana weevils contain flavonoid compounds that can be used as antibacterial agents. This study aims to prove the ethanol extract and fractions from the ethanol extract of kepok banana hump in inhibiting Escherchia coli ESBL (Extended Spectrum Beta Lactamase) bacteria and comparing its inhibitory ability with Chloramphenicol positive control. Kepok banana weevils were macerated with 96% ethanol then dissolved in warm water and fractionated using n-hexane and ethyl acetate solvents. Antibacterial testing was carried out on the extract, n-hexane fraction, ethyl acetate fraction and water fraction of Kepok banana hump. The method used for testing antibacterial activity is the diffusion method. The negative control used DMSO 10%, while the positive control used was chloramphenicol. The results are shown from the radical inhibition zone diameter. Antibacterial statistical analysis used one way ANOVA. The radical inhibition zone of ethanol extract with a concentration of 25% was 6.66 mm, a concentration of 50% was 7 mm, while for a concentration of 100% was 6 mm. The n-hexane fraction at concentrations of 25%, 50% and 100% gave the same radical zone, namely 6 mm. Ethyl acetate fraction with 25% concentration of radical inhibition zone obtained was 16.95 mm, 50% concentration was 13.3 mm while at 100% concentration was 11.16 mm. The concentration of water fraction 25% and 50% obtained 6 mm radical zone, 100% concentration obtained a radical inhibition zone of 6.33 mm. Ethyl acetate fraction with 25% concentration gave a bigger radical inhibition zone against Escherichia coli ESBL, which was 16.95 mm including intermediates, not yet equivalent to the positive control chloramphenicol which had a radical inhibition zone of 31.9 mm (sensitive). The data also supported statistically there was a significant difference (p <0.05) between the ethyl acetate and chloramphenicol fractions.
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