PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK
DAUN LEGETAN (Acmella uliginosa)SECARA
SPEKTROFOTOMETRI UV-Vis
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Suci, Wulandari
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2021-03-02 03:59:35
Abstract :
Indonesia is a country rich in natural resources. Some plants can be used as herbal medicines, one of which is the legeten leaves. Legetan leaves contain flavonoid compounds. Flavonoids have antioxidant properties that acts as a free radical scavenger and it contains a hydroxyl group. This study aims to determine the presence or absence of flavonoid content and determine the total flavonoid leaves contained in legetan leaf extract. Extraction is done using maceration method with 70% ethanol solvent. Extract results are used for qualitative and quantitative analysis.Quercetin used as a standard solution. Qualitative analisys using TLC, Wilstater-Cyanidin method, and qualitative analisys using aqueous NaOH. Quantitative analysis using Visible Spectrophotometry on a wavelength of 428.0 nm and operating time at the 15th minute. The results of qualitative test showed that the extract was positive flavonoids. The average concentrations of total flavonoids was 0,9148% extract with a variation coefficient value of 0,21%.
Indonesia is a country rich in natural resources. Some plants can be used as herbal medicines, one of which is the legeten leaves. Legetan leaves contain flavonoid compounds. Flavonoids have antioxidant properties that acts as a free radical scavenger and it contains a hydroxyl group. This study aims to determine the presence or absence of flavonoid content and determine the total flavonoid leaves contained in legetan leaf extract. Extraction is done using maceration method with 70% ethanol solvent. Extract results are used for qualitative and quantitative analysis.Quercetin used as a standard solution. Qualitative analisys using TLC, Wilstater-Cyanidin method, and qualitative analisys using aqueous NaOH. Quantitative analysis using Visible Spectrophotometry on a wavelength of 428.0 nm and operating time at the 15th minute. The results of qualitative test showed that the extract was positive flavonoids. The average concentrations of total flavonoids was 0,9148% extract with a variation coefficient value of 0,21%.
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