ANALISIS KADAR TOTAL FLAVONOID EKSTRAK
ETANOL DAUN ADAS (Foeniculum vulgare)
SECARA SPEKTROFOTOMETRI VISIBEL
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Alifia, Ni''ma
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2022-02-04 02:08:01
Abstract :
Flavonoid is one the compounds in nature that play a role in increasing body immunity. Sources of flavonoid can be found in fennel leaves. This research aims to determine total flavonoid content in fennel leaves (Foeniculum vulgare) using the Visible Spectrophotometry method. Extraction is done using the maceration method with 96% ethanol solvent. The qualitative analysis of flavonoid using NaOH, Wilstater-cyanidin method, and AlCl3. Quersetin used as standard solution. Quantitative analysis was using colorimetry method with reagent AlCl3 to forms complex compound. Quantitative analysis was continued with Visible Spectrophotometry on operating time at the 28th minute and a wavelength of 430,5 nm. The results of the qualitative analysis showed that the ethanol extract of fennel leaves was positive flavonoids. The average concentrations of total flavonoids of fennel leaves ethanol extract is 99,2 mg QE/g extract with a variation coefficient value of 1,27%.
Flavonoid is one the compounds in nature that play a role in increasing body immunity. Sources of flavonoid can be found in fennel leaves. This research aims to determine total flavonoid content in fennel leaves (Foeniculum vulgare) using the Visible Spectrophotometry method. Extraction is done using the maceration method with 96% ethanol solvent. The qualitative analysis of flavonoid using NaOH, Wilstater-cyanidin method, and AlCl3. Quersetin used as standard solution. Quantitative analysis was using colorimetry method with reagent AlCl3 to forms complex compound. Quantitative analysis was continued with Visible Spectrophotometry on operating time at the 28th minute and a wavelength of 430,5 nm. The results of the qualitative analysis showed that the ethanol extract of fennel leaves was positive flavonoids. The average concentrations of total flavonoids of fennel leaves ethanol extract is 99,2 mg QE/g extract with a variation coefficient value of 1,27%.
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