PENGARUH VARIASI KONSENTRASI SARI PATI BUAH
SUKUN SEBAGAI ALTERNATIF MEDIA SEMI SINTETIK
PADA PERTUMBUHAN JAMUR Candida albicans
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Reza, Yuliana
Subject
R Medicine (General)
Datestamp
2022-04-14 07:39:31
Abstract :
Gold standard examination of Candida sp. is a culture test. Potato Dextrose Agar (PDA) is a medium that can be used to culture the fungus Candida albicans. PDA media are manufactured in a ready-to-use dosage form, are hygroscopic, and are only obtained at certain places. The abundance of natural resources in Indonesia encourages researchers to take advantage of the wealth of biological natural resources to create alternative semi-synthetic media for mushroom growth from breadfruit. The purpose of this study was to determine the effective concentration of alternative semi-synthetic media of breadfruit essence as a growth medium for the fungus Candida albicans. This research is an experimental study by inoculating Candida albicans with the spread plate method. Observations were made after the media was incubated for 72 hours. Observations were made macroscopically and confirmed microscopically. Macroscopic observation of the number of colonies was carried out by the Shapiro Wilk test and the One Way Anova test. The results showed that the average growth of the number of colonies on PDA media was 15.50, while for the alternative breadfruit media the average number of colonies was 100% concentration 24.50, 90% concentration 19.75, 80% concentration 19.50, 70% concentration 16.00, 60% concentration 15.50. Shapiro Wilk's normality test obtained p value> 0.05 then the distribution of the data obtained was normal. The One Way Anova test showed that the homogeneity test results were p> 0.05, so the data obtained in this study were homogeneous and evenly distributed. The results of the ANOVA test obtained a p value <0.05, so H0 in this study was rejected and H1 was accepted, namely there was a significant difference in the average growth of fungi in the media of breadfruit essence. The conclusion from this research is that the concentration of 60% breadfruit essence is the effective concentration for growing Candida albicans because the results obtained are close to control.
Gold standard examination of Candida sp. is a culture test. Potato Dextrose Agar (PDA) is a medium that can be used to culture the fungus Candida albicans. PDA media are manufactured in a ready-to-use dosage form, are hygroscopic, and are only obtained at certain places. The abundance of natural resources in Indonesia encourages researchers to take advantage of the wealth of biological natural resources to create alternative semi-synthetic media for mushroom growth from breadfruit. The purpose of this study was to determine the effective concentration of alternative semi-synthetic media of breadfruit essence as a growth medium for the fungus Candida albicans. This research is an experimental study by inoculating Candida albicans with the spread plate method. Observations were made after the media was incubated for 72 hours. Observations were made macroscopically and confirmed microscopically. Macroscopic observation of the number of colonies was carried out by the Shapiro Wilk test and the One Way Anova test. The results showed that the average growth of the number of colonies on PDA media was 15.50, while for the alternative breadfruit media the average number of colonies was 100% concentration 24.50, 90% concentration 19.75, 80% concentration 19.50, 70% concentration 16.00, 60% concentration 15.50. Shapiro Wilk's normality test obtained p value> 0.05 then the distribution of the data obtained was normal. The One Way Anova test showed that the homogeneity test results were p> 0.05, so the data obtained in this study were homogeneous and evenly distributed. The results of the ANOVA test obtained a p value <0.05, so H0 in this study was rejected and H1 was accepted, namely there was a significant difference in the average growth of fungi in the media of breadfruit essence. The conclusion from this research is that the concentration of 60% breadfruit essence is the effective concentration for growing Candida albicans because the results obtained are close to control.
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