IDENTIFIKASI DAN PENETAPAN KADAR FLAVONOID TOTAL
EKSTRAK DAN FRAKSI-FRAKSI BUNGA PEPAYA JANTAN
(Carica papaya L.)
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Dilla, Nur Pratiwi
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2022-04-14 07:40:45
Abstract :
Male papaya flower (Carica papaya L.) is one part of the plant that has not been explored much for content and properties. One of the metabolites in papaya flowers is flavonoids, flavonoid compounds have antioxidant, antiaging activity. This study to identify flavonoid compounds and determine flavonoid content from ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction. This study used male papaya flowers extracted with 70% ethanol using maceration method for 3 days and remaceration for 2 day, then fractionated. Determination flavonoid content using aluminum chloride colorimetric method, TLC with n-butanol:acetic acid:water (4:1:5) as mobile phase and UV-Vis flavonoid identification based on the presence of bands cinnamoyl and benzoyl. Ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction had total flavonoid content equivalent to quercetin of 0,6805 ± 0,0045 %QE, 0,4178 ± 0,0058 %QE, 0,5411 ± 0,0052% QE and 0,5207 ± 0,0007 %QE. The Rf value of the sample at 0.9375 indicates the presence of flavonoid compounds. Identification of flavonoid ethanol extract, ethyl acetate fraction and water fraction obtained cinnamoyl and benzoyl are a substituted flavonoid 3-OH flavonoid, while n-hexane fraction obtained cinnamoyl and benzoyl are flavonoids of the free flavonol 3-OH type.
Male papaya flower (Carica papaya L.) is one part of the plant that has not been explored much for content and properties. One of the metabolites in papaya flowers is flavonoids, flavonoid compounds have antioxidant, antiaging activity. This study to identify flavonoid compounds and determine flavonoid content from ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction. This study used male papaya flowers extracted with 70% ethanol using maceration method for 3 days and remaceration for 2 day, then fractionated. Determination flavonoid content using aluminum chloride colorimetric method, TLC with n-butanol:acetic acid:water (4:1:5) as mobile phase and UV-Vis flavonoid identification based on the presence of bands cinnamoyl and benzoyl. Ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction had total flavonoid content equivalent to quercetin of 0,6805 ± 0,0045 %QE, 0,4178 ± 0,0058 %QE, 0,5411 ± 0,0052% QE and 0,5207 ± 0,0007 %QE. The Rf value of the sample at 0.9375 indicates the presence of flavonoid compounds. Identification of flavonoid ethanol extract, ethyl acetate fraction and water fraction obtained cinnamoyl and benzoyl are a substituted flavonoid 3-OH flavonoid, while n-hexane fraction obtained cinnamoyl and benzoyl are flavonoids of the free flavonol 3-OH type.
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