PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL DAN
FRAKSI ETIL ASETAT DAUN JERUK NIPIS (Citrus aurantiifolia
(Christm. & Panz.) Swingle.) SERTA UJI AKTIVITAS ANTIBAKTERI
TERHADAP Salmonella typhi
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Isnaini, Pratiwi
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2022-04-14 07:41:19
Abstract :
Lime leaves (Citrus aurantiifolia) contain flavonoid compounds that have the potential as antibacterial. This study aims to determine the total flavonoid content and antibacterial activity of Salmonella typhi between the ethanol extract and the ethyl acetate fraction of lime leaf (Citrus aurantiifolia). In this study, lime leaves were extracted by maceration method using 70% ethanol solvent then dissolved in warm water and fractionated using n-hexane and ethyl acetate as solvents. Determination of total flavonoid levels using UV-Vis spectrophotometry and then antibacterial test using the diffusion method. The results of the study were to determine the total flavonoid content of the ethanol extract and the ethyl acetate fraction of lime leaf, respectively, at 1.0324 w/w QE and 1.2702 w/w QE. Statistical analysis of the Independent Samples T-Test showed that the total flavonoid content in the ethanol extract and the ethyl acetate fraction of lime leaves had a significant difference (p=0.000 (p<0.05)). Statistical results of the inhibitory power of ethanol extract and ethyl acetate fraction were able to inhibit Salmonella typhi bacteria at a concentration of 100% with an inhibition zone diameter of 10.4 mm and 12.63 mm in the resistant category which had a significant difference (p<0.05) with DMSO 10% negative control. These results were not equivalent to the positive control of chloramphenicol 30 g with an inhibition zone of 34.5 mm.
Lime leaves (Citrus aurantiifolia) contain flavonoid compounds that have the potential as antibacterial. This study aims to determine the total flavonoid content and antibacterial activity of Salmonella typhi between the ethanol extract and the ethyl acetate fraction of lime leaf (Citrus aurantiifolia). In this study, lime leaves were extracted by maceration method using 70% ethanol solvent then dissolved in warm water and fractionated using n-hexane and ethyl acetate as solvents. Determination of total flavonoid levels using UV-Vis spectrophotometry and then antibacterial test using the diffusion method. The results of the study were to determine the total flavonoid content of the ethanol extract and the ethyl acetate fraction of lime leaf, respectively, at 1.0324 w/w QE and 1.2702 w/w QE. Statistical analysis of the Independent Samples T-Test showed that the total flavonoid content in the ethanol extract and the ethyl acetate fraction of lime leaves had a significant difference (p=0.000 (p<0.05)). Statistical results of the inhibitory power of ethanol extract and ethyl acetate fraction were able to inhibit Salmonella typhi bacteria at a concentration of 100% with an inhibition zone diameter of 10.4 mm and 12.63 mm in the resistant category which had a significant difference (p<0.05) with DMSO 10% negative control. These results were not equivalent to the positive control of chloramphenicol 30 g with an inhibition zone of 34.5 mm.
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