UJI AKTIVITAS ANTIBAKTERI FRAKSI ETIL ASETAT DAUN SIRIH
HIJAU (Piper betle L.) TERHADAP Staphylococcus epidermidis DAN
Pseudomnas aeruginosa
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Maryani, Maryani
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2022-04-14 07:41:37
Abstract :
Bacterial infections are usually caused by Staphylococcus epidermidis and Pseudomonas aeruginosa which can cause swelling. Alternative medicine can use herbal plants such as green betel which contains flavonoid compounds, tannins, saponins, alkaloids. The purpose of this study was to determine whether green betel leaf has antibacterial activity that provides inhibition against Staphylococcus epidermidis and Pseudomonas aeruginosa. The antibacterial activity test of the ethyl acetate fraction of green betel leaf by disc diffusion method was observed to observe the diameter of the inhibition zone formed around the disc. Observation of the concentration of the ethyl acetate fraction using a caliper, concentrations of 25%, 50% and 75% were compared with the negative control, namely DMSO 10% and the positive control, namely tetracycline. Research result at the minimum concentration of the ethyl acetate fraction of green betel leaf, which is 25%, it was able to inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa bacteria which showed a higher value than the bnegative control significant (P<0,05). Anova table of Staphylococcus epidermidis and Pseudomonas aeruginosa in column significant obtained P value = 0,00 so at the level of significance =0,05 so that there is a significant difference in concentration with positive control.
Bacterial infections are usually caused by Staphylococcus epidermidis and Pseudomonas aeruginosa which can cause swelling. Alternative medicine can use herbal plants such as green betel which contains flavonoid compounds, tannins, saponins, alkaloids. The purpose of this study was to determine whether green betel leaf has antibacterial activity that provides inhibition against Staphylococcus epidermidis and Pseudomonas aeruginosa. The antibacterial activity test of the ethyl acetate fraction of green betel leaf by disc diffusion method was observed to observe the diameter of the inhibition zone formed around the disc. Observation of the concentration of the ethyl acetate fraction using a caliper, concentrations of 25%, 50% and 75% were compared with the negative control, namely DMSO 10% and the positive control, namely tetracycline. Research result at the minimum concentration of the ethyl acetate fraction of green betel leaf, which is 25%, it was able to inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa bacteria which showed a higher value than the bnegative control significant (P<0,05). Anova table of Staphylococcus epidermidis and Pseudomonas aeruginosa in column significant obtained P value = 0,00 so at the level of significance =0,05 so that there is a significant difference in concentration with positive control.
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