IDENTIFIKASI DAN PENENTUAN KADAR FLAVONOID TOTAL
EKSTRAK DAN FRAKSI-FRAKSI DAUN UMBI BIT MERAH (Beta
vulgaris var. Rubra (L.) Moq.)
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Noor, Anisa Mahanani
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2022-04-14 07:41:53
Abstract :
Beetroot leaf (Beta vulgaris L) has many benefits, one of which is consumed as a side dish. In addition, beetroot leaf have pharmacological activities such as hepatoprotective and antioxidant. This activity is influenced by the role of secondary metabolites, one of which is flavonoid compounds. This study aims to determine the levels of flavonoids in ethanol extract and beetroot leaf fractions. In this study, the beetroot leaf used was red beetroot (Beta vulgaris var. Rubra (L.) Moq.) were extracted with 70% ethanol using maceration method for 3 days and remaceration for 1 day, then proceed with graded fractionation. The extracts and fractions of beetroot leaves were determined for total flavonoid content using the alumunium chloride colorimetric method and identification of flavonoids by UV-Vis spectroscopy. The results of the determination of flavonoid levels showed that the ethyl acetate fraction of red beetroot leaves contained the highest flavonoid content of 1,8349±0.0302 %QE, followed by the n-hexane fraction of 1,6736±0,0275 %QE, ethanol extract of 1,6736±0,0033 %QE and the water fraction is 1,6659±0,0302 %QE. Identification of flavonoids based on spectrum analysis of UV-Vis spectroscopy readings showed the presence of flavonoids of the flavonol type contained in the extract and fractions of red beetroot leaves.
Beetroot leaf (Beta vulgaris L) has many benefits, one of which is consumed as a side dish. In addition, beetroot leaf have pharmacological activities such as hepatoprotective and antioxidant. This activity is influenced by the role of secondary metabolites, one of which is flavonoid compounds. This study aims to determine the levels of flavonoids in ethanol extract and beetroot leaf fractions. In this study, the beetroot leaf used was red beetroot (Beta vulgaris var. Rubra (L.) Moq.) were extracted with 70% ethanol using maceration method for 3 days and remaceration for 1 day, then proceed with graded fractionation. The extracts and fractions of beetroot leaves were determined for total flavonoid content using the alumunium chloride colorimetric method and identification of flavonoids by UV-Vis spectroscopy. The results of the determination of flavonoid levels showed that the ethyl acetate fraction of red beetroot leaves contained the highest flavonoid content of 1,8349±0.0302 %QE, followed by the n-hexane fraction of 1,6736±0,0275 %QE, ethanol extract of 1,6736±0,0033 %QE and the water fraction is 1,6659±0,0302 %QE. Identification of flavonoids based on spectrum analysis of UV-Vis spectroscopy readings showed the presence of flavonoids of the flavonol type contained in the extract and fractions of red beetroot leaves.
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