PENETAPAN KADAR FENOLIK TOTAL EKSTRAK AIR DAUN KELOR
(Moringa oleifera Lamk) SECARA SPEKTROFOTOMETRI UV-Vis
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Avita, Hosiana
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2020-12-23 02:27:31
Abstract :
Moringa oleifera is one of the Indonesian plant containing phenolic compounds. A phenolic compound that has the ability to change or reduce free radicals. Flavonoids are one of phenolic compounds have a variety antioxidant activity, anti-inflammatory, hypo-allergenic, hepatoprotective, antithrombotic, antiviral and anticarcinogenic activity. The purpose of this study was to determine the total phenolic content of Moringa oleifera leaves qualitatively and to determine the total phenolic content of the water extract of Moringa oleifera leaves quantitatively. Samples were extracted with infundation methode using water. Infusa obtained are evaporated on water bath. Qualitative test was conducted using a preliminary test their phenolic compounds and Thin Layer Chromatography the stationary phase silica gel F 254 and a mobile phase of butanol: acetic acid: water (5: 1: 4). Determination of total phenolic content using UV-Vis Spectrophotometer with the Folin-Ciocalteu reagent at a wavelength of 645.0 nm and at OT 34-37 minutes. The conclusion of this study are Moringa oleifera leave positif contain phenolic compounds with total phenolic content of the extract 11,234x10-3 ± 2,571x10-6% w / w GAE.
Moringa oleifera is one of the Indonesian plant containing phenolic compounds. A phenolic compound that has the ability to change or reduce free radicals. Flavonoids are one of phenolic compounds have a variety antioxidant activity, anti-inflammatory, hypo-allergenic, hepatoprotective, antithrombotic, antiviral and anticarcinogenic activity. The purpose of this study was to determine the total phenolic content of Moringa oleifera leaves qualitatively and to determine the total phenolic content of the water extract of Moringa oleifera leaves quantitatively. Samples were extracted with infundation methode using water. Infusa obtained are evaporated on water bath. Qualitative test was conducted using a preliminary test their phenolic compounds and Thin Layer Chromatography the stationary phase silica gel F 254 and a mobile phase of butanol: acetic acid: water (5: 1: 4). Determination of total phenolic content using UV-Vis Spectrophotometer with the Folin-Ciocalteu reagent at a wavelength of 645.0 nm and at OT 34-37 minutes. The conclusion of this study are Moringa oleifera leave positif contain phenolic compounds with total phenolic content of the extract 11,234x10-3 ± 2,571x10-6% w / w GAE.
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