PENETAPAN KADAR FLAVONOID TOTAL DAN UJI AKTIVITAS
ANTIBAKTERI EKSTRAK ETANOL 70% DAN FRAKSI ETIL ASETAT
KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) TERHADAP
BAKTERI Escherichia coli DAN Staphylococcus aureus
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Novitasari, Novitasari
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2022-04-14 07:41:59
Abstract :
Red dragon fruit peel (Hylocereus polyrhizus) of content flavonoid compound that are beneficialas antibacterial. The research determine of total flavonoid content and antibacterial activities of extracts ethanol and fractions ethyl acetate red dragon fruit peel against Escherichia coli ans Staphylococcus aureus. Red dragon fruit peel of extracted using 70% ethanol solvent of maceration method and fractionatedusing n-hexane and ethyl acetat solvent. Determination of flavonoids content using aluminium chloride method with UVVis spectrophotometry and the results were analyzed using the Independent Sample T-Test. Antibacterial activities using the diffusion disk method with concentrations of 25%, 50% and 75%, using negative control a DMSO 10% and positive control a Ciprofoxacin. The result of identification flavonoids compounds indicate red dragon fruit peel extracts ethanol and fractions ethyl acetat contain flavonoids compounds. The determination of total flavonoid contained in extracts ethanol and fraction ethyl acetat of 8,773 %QE ± 0,122 and 11,596 %QE ± 0,089. There was a significant difference in the average total flavonoid content in 70% ethanol extract with the ethyl acetate fraction of red dragon fruit peel (Hylocereus polyrhizus) (p<0.05). The average results of the antibacterial inhibition zones of the ethanol extract of red dragon fruit peel with concentrations of 25%, 50% and 75% against Escherichia coli bacteria were 7.3 mm, 8.3 mm and 9.5 mm, while the ethyl acetate fraction of red dragon fruit peel concentrations of 25%, 50% and 75% against Escherichia coli bacteria obtained an average of 8 mm, 9.5 mm and 11.2 mm. The average results obtained on the ethanol extract of red dragon fruit peel with concentrations of 25%, 50% and 75% against Staphylococcus aureus bacteria were 7.6 mm, 8.6 mm, 9.8 mm, while for the zone average results Inhibit the ethyl acetate fraction of red dragon fruit peel against Staphylococcus aureus at concentrations of 25%, 50% and 75%, namely 8 mm, 9.4 mm and 11.9 mm. Results Based on the Post Hoc Test, the ethanol extract and the ethyl acetate fraction of red dragon fruit peel had antibacterial activity against Escherichia coli and Staphylococcus aureus because the zone of inhibition was significantly different (p < 0.05) with the negative control (DMSO 10%). The results of the inhibition zone obtained by the ethanol extract and the red dragon fruit peel fraction were not equivalent to the inhibition zone obtained in the positive control of Ciprofloxacin against Escherichia coli and Staphylococcus aureus bacteria, namely 36 mm and 26.66 mm
Red dragon fruit peel (Hylocereus polyrhizus) of content flavonoid compound that are beneficialas antibacterial. The research determine of total flavonoid content and antibacterial activities of extracts ethanol and fractions ethyl acetate red dragon fruit peel against Escherichia coli ans Staphylococcus aureus. Red dragon fruit peel of extracted using 70% ethanol solvent of maceration method and fractionatedusing n-hexane and ethyl acetat solvent. Determination of flavonoids content using aluminium chloride method with UVVis spectrophotometry and the results were analyzed using the Independent Sample T-Test. Antibacterial activities using the diffusion disk method with concentrations of 25%, 50% and 75%, using negative control a DMSO 10% and positive control a Ciprofoxacin. The result of identification flavonoids compounds indicate red dragon fruit peel extracts ethanol and fractions ethyl acetat contain flavonoids compounds. The determination of total flavonoid contained in extracts ethanol and fraction ethyl acetat of 8,773 %QE ± 0,122 and 11,596 %QE ± 0,089. There was a significant difference in the average total flavonoid content in 70% ethanol extract with the ethyl acetate fraction of red dragon fruit peel (Hylocereus polyrhizus) (p<0.05). The average results of the antibacterial inhibition zones of the ethanol extract of red dragon fruit peel with concentrations of 25%, 50% and 75% against Escherichia coli bacteria were 7.3 mm, 8.3 mm and 9.5 mm, while the ethyl acetate fraction of red dragon fruit peel concentrations of 25%, 50% and 75% against Escherichia coli bacteria obtained an average of 8 mm, 9.5 mm and 11.2 mm. The average results obtained on the ethanol extract of red dragon fruit peel with concentrations of 25%, 50% and 75% against Staphylococcus aureus bacteria were 7.6 mm, 8.6 mm, 9.8 mm, while for the zone average results Inhibit the ethyl acetate fraction of red dragon fruit peel against Staphylococcus aureus at concentrations of 25%, 50% and 75%, namely 8 mm, 9.4 mm and 11.9 mm. Results Based on the Post Hoc Test, the ethanol extract and the ethyl acetate fraction of red dragon fruit peel had antibacterial activity against Escherichia coli and Staphylococcus aureus because the zone of inhibition was significantly different (p < 0.05) with the negative control (DMSO 10%). The results of the inhibition zone obtained by the ethanol extract and the red dragon fruit peel fraction were not equivalent to the inhibition zone obtained in the positive control of Ciprofloxacin against Escherichia coli and Staphylococcus aureus bacteria, namely 36 mm and 26.66 mm
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