GAMBARAN KADAR BILIRUBIN DIREK YANG DIPERIKSA
SEGERA DAN DITUNDA PADA SUHU 20-25o C SELAMA DUA HARI
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Afifah, Eka Santi Agung
Subject
R Medicine (General)
Datestamp
2022-04-14 07:43:12
Abstract :
Laboratory tests are very important to help establish the diagnosis of the disease. Control of pre-analytical, analytical, and post-analytic can provide accurate and reliable laboratory test results. The biggest source of error can occur in the preanalytic stage, including due to improper sample storage and inspection delays. Inspection delays can occur due to equipment failure or the large number of samples being examined. Bilirubin is a yellow pigment derived from the breakdown of heme from hemoglobin in the breakdown of erythrocytes by reticuloendothelial cells. Meanwhile, direct bilirubin is free bilirubin that is found in the liver and no longer binds to albumin. Factors that affect the increase in direct bilirubin levels are the delay in examination which can cause protein denaturation. The purpose of this study was to determine the results of direct bilirubin levels which were immediately checked and postponed. This study uses an experimental research design. This research was conducted at the Independent Diagnostic Center Laboratory and the time of the study was carried out in January-December 2021. The sample of this research was 15 students of class 3A2 of DIII Medical Laboratory Technology National STIKES. The results showed that the normality test was without a delay of 0.519 and a delay of 0.225. The results are normally distributed because the sig value > 0.05. Then continued with the paired T test, the result was 0.000. The conclusion of this study is that there is an effect of delaying serum samples on direct bilirubin levels.
Laboratory tests are very important to help establish the diagnosis of the disease. Control of pre-analytical, analytical, and post-analytic can provide accurate and reliable laboratory test results. The biggest source of error can occur in the preanalytic stage, including due to improper sample storage and inspection delays. Inspection delays can occur due to equipment failure or the large number of samples being examined. Bilirubin is a yellow pigment derived from the breakdown of heme from hemoglobin in the breakdown of erythrocytes by reticuloendothelial cells. Meanwhile, direct bilirubin is free bilirubin that is found in the liver and no longer binds to albumin. Factors that affect the increase in direct bilirubin levels are the delay in examination which can cause protein denaturation. The purpose of this study was to determine the results of direct bilirubin levels which were immediately checked and postponed. This study uses an experimental research design. This research was conducted at the Independent Diagnostic Center Laboratory and the time of the study was carried out in January-December 2021. The sample of this research was 15 students of class 3A2 of DIII Medical Laboratory Technology National STIKES. The results showed that the normality test was without a delay of 0.519 and a delay of 0.225. The results are normally distributed because the sig value > 0.05. Then continued with the paired T test, the result was 0.000. The conclusion of this study is that there is an effect of delaying serum samples on direct bilirubin levels.
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