PENETAPAN KADAR FLAVONOID TOTAL DARI FRAKSI -
FRAKSI DAUN INSULIN (Smallanthus sonchifolius) SECARA
SPEKTROFOTOMETRI UV-VIS
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Institusion
Sekolah Tinggi Ilmu Kesehatan Nasional
Author
Fatma, Berliana
Subject
RN Pharmacy and Pharmaceutical Mathematics
Datestamp
2022-07-05 02:09:56
Abstract :
Insulin leaves are one of the plants that are easily found in indonesia and have many benefit. Insulin leaves contain bioactive compounds such as proteins, lipids, fiber and flavonoids. Flavonoids are compounds that can be used as antibacterial . this study aims to determine the total flavonoid content of the fraction of ethyl acetate, n-hexane and insulin leaf water and knowing the highest flavonoid content from the ethyl acetate, n-Hexane, and water fractions. The method used in this research is the liquid – liquid fraction namely the ethyl acetate, n-hexane, and water fractions. Quantitative analysis for determination of total flavonoid content of insulin leaf fractions using UV-Vis apectrophotometry at wavelength of 431,5 nm and Operating Time 33 minutes. Based on the results of the research, the determination of the total flavonoid content of the insulin leaf fractions that has been obtained in the ethyl acetate fraction is 21,0547 mgQE/100ml, the n-hexane fraction is 20,1367 mgQE/100ml and water fraction is 18,31738 mgQE/100ml and the highest total flavonoid content is obtained in the ethyl acetate fraction.
Insulin leaves are one of the plants that are easily found in indonesia and have many benefit. Insulin leaves contain bioactive compounds such as proteins, lipids, fiber and flavonoids. Flavonoids are compounds that can be used as antibacterial . this study aims to determine the total flavonoid content of the fraction of ethyl acetate, n-hexane and insulin leaf water and knowing the highest flavonoid content from the ethyl acetate, n-Hexane, and water fractions. The method used in this research is the liquid – liquid fraction namely the ethyl acetate, n-hexane, and water fractions. Quantitative analysis for determination of total flavonoid content of insulin leaf fractions using UV-Vis apectrophotometry at wavelength of 431,5 nm and Operating Time 33 minutes. Based on the results of the research, the determination of the total flavonoid content of the insulin leaf fractions that has been obtained in the ethyl acetate fraction is 21,0547 mgQE/100ml, the n-hexane fraction is 20,1367 mgQE/100ml and water fraction is 18,31738 mgQE/100ml and the highest total flavonoid content is obtained in the ethyl acetate fraction.
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