Karakterisasi Paired-Like Homeodomain Transcription Factor 2 (PITX2) sebagai Penanda Molekuler untuk Prediksi Asimetri Mandibula dalam Arah Vertikal (kajian genomik, Lingkungan, dan Radiografi Panoramik)
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Institusion
Universitas Sumatera Utara
Author
Sofyanti, Ervina
Subject
PITX2
Datestamp
2018-12-27 03:58:47
Abstract :
Background: The complexity of mandibular asymmetry development in malocclusion explains in part why most treatment approaches for malocclusion are directed to the symptoms rather than to etiology. The understanding of genetic and environmental factors interaction is fundamental to the evidence based practice of orthodontics to determine what the best treatment plan is for each patient in the era of truly personalized orthodontics. Pituitary Homeobox Protein Type-2 (PITX2), an active gene in Nodal Signalling pathway that involves as transcription factor in regulating the symmetrical of vertebrae from embriology to post-natal. Aims: This study was conducted to analyze PITX2 gene as a molecular in predict vertical mandible asymmetry based on genomic, environmental, and panoramic radiograph. Method : This research was an observational analytic with case-control study of 129 outcoming orthodontics patients of Dental hospital, Faculty of Denstistry Universitas Sumatera Utara ranged between 11 ? 25 years old. Determination of vertical mandibular symmetry based on Kjellberg?s technique on panoramic radiographs before treatment. Dental record was collected and DNA isolation by buccal swab was taken. The difference between symmetrical and asymmetrical group used Chi-squared test and continue with Odds ratio measurement. DNA material was obtained using buccal swabs, followed by Polymerase Chain Reaction (PCR) and Sanger sequencing with ChromasPro 2.13 software (Technelysium, Queensland, Australia) and then compared to archival data from gene bank number ENSG00000164093 (www.ensembl.org). Result : There was a significant difference in trunk posture symmetry based on vertical mandibular symmetry (p=0.047). The characteristics of PITX2 gene showed three polymophism based on sequensing and showed three polymorphism (rs72554076, rs761511445, rs372257787) in 4?UTR from 16 samples. We found compound mutation and mutation in 46+80 C>A that was never reported in gene bank database which showed condylar height asymmetry. Conclusion : Variability in PITX2 DNA sequence, asymmetry of trunk posture and mandibular asymmetry in vertical directions based on panoramic radiographic should be required in the personal orthodontic treatment approach.
Background: The complexity of mandibular asymmetry development in malocclusion explains in part why most treatment approaches for malocclusion are directed to the symptoms rather than to etiology. The understanding of genetic and environmental factors interaction is fundamental to the evidence based practice of orthodontics to determine what the best treatment plan is for each patient in the era of truly personalized orthodontics. Pituitary Homeobox Protein Type-2 (PITX2), an active gene in Nodal Signalling pathway that involves as transcription factor in regulating the symmetrical of vertebrae from embriology to post-natal. Aims: This study was conducted to analyze PITX2 gene as a molecular in predict vertical mandible asymmetry based on genomic, environmental, and panoramic radiograph. Method : This research was an observational analytic with case-control study of 129 outcoming orthodontics patients of Dental hospital, Faculty of Denstistry Universitas Sumatera Utara ranged between 11 ? 25 years old. Determination of vertical mandibular symmetry based on Kjellberg?s technique on panoramic radiographs before treatment. Dental record was collected and DNA isolation by buccal swab was taken. The difference between symmetrical and asymmetrical group used Chi-squared test and continue with Odds ratio measurement. DNA material was obtained using buccal swabs, followed by Polymerase Chain Reaction (PCR) and Sanger sequencing with ChromasPro 2.13 software (Technelysium, Queensland, Australia) and then compared to archival data from gene bank number ENSG00000164093 (www.ensembl.org). Result : There was a significant difference in trunk posture symmetry based on vertical mandibular symmetry (p=0.047). The characteristics of PITX2 gene showed three polymophism based on sequensing and showed three polymorphism (rs72554076, rs761511445, rs372257787) in 4?UTR from 16 samples. We found compound mutation and mutation in 46+80 C>A that was never reported in gene bank database which showed condylar height asymmetry. Conclusion : Variability in PITX2 DNA sequence, asymmetry of trunk posture and mandibular asymmetry in vertical directions based on panoramic radiographic should be required in the personal orthodontic treatment approach.
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