Institusion
Universitas Sumatera Utara
Author
Khoiriah, Nikmatul (STUDENT ID : 050805017)
(LECTURER ID : 0024056503)
(LECTURER ID : 0023056005)
Subject
Plant tissue culture
Datestamp
2022-12-09 07:12:17
Abstract :
The study on ? The Leaf Tissue Culture of andaliman (Zanthoxylum
acanthopodium DC.) with induction of EMS (Ethyl Methana Sulphonate)? has
been done in Plant Tissue Culture Laboratory of Biologi Department at Faculty of
Mathematics and Natural Science North Sumatera University from March until
October 2009. The objective of the research was to obtain the effect of concentration
and submertion time of EMS to increase growth the leaf culture. The design of the
research is complete rendomized design factorial with 2 factors, those are 5 levels of
EMS 0,0; 0,05; 0,10; 0,15; and 0,20%, combined with time of submertion of 30, 60,
and 90 minutes. The statistic analysis showed that the raising of concentration anda
time of submertion of EMS caused decrease of calli fresh weight. The higgest of fresh
weight found in C1T2 (0,05% EMS and 60 minute submertion of time).