Abstract :
This research aims to determine the quality of frozen semen of post thawing bali cattle at different distances and times with the observed parameters of motility, viability and abnormalities of spermatozoa. The research method used is experimental laboratory. This research used frozen semen of Bali cattle at the Gorontalo Provincial Agriculture Office whose distribution came from BBIB Singosari. The research design used a factorial completely randomized. The data obtained were analyzed using two-way ANOVA. The highest mean percentage of spermatozoa motility was found at a distance of 10 cm for 15 seconds, the highest mean percentage of spermatozoa viability was found at a distance of 15 cm for 20 seconds, the highest mean percentage of spermatozoa abnormalities was found at a distance of 20 cm for 20 seconds post thawing. The conclusion indicates the distance and time of transfer of raw raw materials from the surface of liquid nitrogen did not affect the quality of frozen semen (motility, viability and spermatozoa abnormalities ) of post thawing bali cattle but were suitable for AI in the field.