IDENTIFIKASI VIRAL NERVOUS NECROSIS (VNN) DENGAN METODE POLYMERASE CHAIN REACTION (PCR) PADA IKAN KERAPU GENUS VARIOLA DI PERAIRAN GORONTALO
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Institusion
Universitas Muhammadiyah Gorontalo
Author
Nurkhozin, Ahmad Ainul
Subject
SH Aquaculture. Fisheries. Angling
Datestamp
2023-02-27 01:28:09
Abstract :
Grouper (Family Serranidae) is a betanodavirus host that infects tropical, subtropical, or cold climate fish species. Betanodaviruses are of major concern in marine species and freshwater fish. The disease caused by this virus is commonly known as Viral Nervous Necrosis (VNN) or Viral Encephalopathy and Retinopathy (VER) which is characterized by vacuolation and necrosis in brain cells, retina, and spinal cord and causes death of up to 100% in larvae and juveniles, and can causing significant losses in older fish. Overall, betanodaviruses have been detected in more than 160 species of fish and several molluscs, both as susceptible hosts and as carriers. Transmission can be through gametes (eggs or sperm) or feces. This research method uses descriptive quantitative observational research methods. The data calculation method used is to determine the prevalence of VNN. Lunar tailed grouper (Variola sp.) samples were taken from sea waters in Gorontalo Province. The research was conducted from October to December 2022. Sampling was carried out once at each of two different locations. The number of fish samples used for VNN testing using the PCR (Polymerase Chain Reaction) method was 22 fish. The test results showed that the prevalence of VNN in Tomini Bay was 16.66% and the prevalence in Kwandang Bay was 10%. It is assumed that the reliability level of the test is 95% (ninety five percent). It was concluded the prevalence of VNN from Tomini Bay was higher than the prevalence from Kwandang Bay.
Grouper (Family Serranidae) is a betanodavirus host that infects tropical, subtropical, or cold climate fish species. Betanodaviruses are of major concern in marine species and freshwater fish. The disease caused by this virus is commonly known as Viral Nervous Necrosis (VNN) or Viral Encephalopathy and Retinopathy (VER) which is characterized by vacuolation and necrosis in brain cells, retina, and spinal cord and causes death of up to 100% in larvae and juveniles, and can causing significant losses in older fish. Overall, betanodaviruses have been detected in more than 160 species of fish and several molluscs, both as susceptible hosts and as carriers. Transmission can be through gametes (eggs or sperm) or feces. This research method uses descriptive quantitative observational research methods. The data calculation method used is to determine the prevalence of VNN. Lunar tailed grouper (Variola sp.) samples were taken from sea waters in Gorontalo Province. The research was conducted from October to December 2022. Sampling was carried out once at each of two different locations. The number of fish samples used for VNN testing using the PCR (Polymerase Chain Reaction) method was 22 fish. The test results showed that the prevalence of VNN in Tomini Bay was 16.66% and the prevalence in Kwandang Bay was 10%. It is assumed that the reliability level of the test is 95% (ninety five percent). It was concluded the prevalence of VNN from Tomini Bay was higher than the prevalence from Kwandang Bay.
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