AKTIVITAS INHIBISI ENZIM ALFA-GLUKOSIDASE DARI
EKSTRAK BUNGA KERSEN (Muntingia calabura L.)
SECARA IN VITRO
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Institusion
Universitas Muslim Indonesia
Author
Azzahra, Resqinah
Maryam, St.
Tahir, Masdiana
Maryam, St.
Tahir, Masdiana
Subject
RS Pharmacy and materia medica
Datestamp
2024-04-18 02:10:19
Abstract :
RESQINAH AZZAHRA. Aktivitas Inhibisi Enzim Alfa-Glukosidase Dari Ekstrak Bunga Kersen (Muntingia calabura L.) Secara In Vitro. (Dibimbing oleh St. Maryam dan Masdiana Tahir). Tumbuhan kersen merupakan salah satu tumbuhan yang sangat potensial untuk dimanfaatkan karena memiliki beberapa kandungan bioaktif yang bermanfaat untuk kesehatan salah satunya ialah flavonoid. Penelitian ini bertujuan untuk mengetahui aktivitas inhibisi enzim alfa-glukosidase dari ekstrak etanol bunga kersen (Muntingia calabura L.) secara in vitro menggunakan alat instrument Microplate reader. Metode penelitian ini diawali dengan penyiapan sampel dengan cara dimaserasi menggunakan pelarut etanol 96% lalu dilakukan uji penghambatan aktivitas enzim alfaglukosidase menggunakan substrat p-nitrofenil-?-D-glukopiranosida (PNPG) dan akarbosa sebagai pembanding. Potensi suatu senyawa dalam menghambat aktivitas enzim dapat diketahui melalui perhitungan IC50. Hasil penelitian menunjukkan bahwa ekstrak etanol bunga kersen (Muntingia calabura L.) memiliki nilai IC50 sebesar 158,262 ?g/mL dan nilai IC50 akarbosa sebesar 0,256 ?g/mL. Kata Kunci: Bunga kersen (Muntingia calabura L.), alfa-glukosidase, IC50 RESQINAH AZZAHRA. In Vitro Inhibition Assay of Alpha-Glucosidase of Jamaica Cherry Extract (Muntingia calabura L.). (Supervised by St. Maryam and Masdiana Tahir). The Muntingia calabura L., commonly referred to as Jamaica cherry, is recognized for its significant potential in therapeutic applications, primarily attributed to its rich bioactive constituents, notably flavonoids. The primary objective of this study was to elucidate the inhibitory activities of alphaglucosidase enzyme derived from the ethanol extract of Jamaica cherry using the microplate reader instrument. The experimental methodology entailed an initial phase of sample preparation, wherein Jamaica cherry underwent maceration with a 96% ethanol. Subsequent phases involved assessing the inhibitory potential of alpha-glucosidase, employing pnitrophenyl- ?-D-glucopyranoside (PNPG) as the substrate, with acarbose serving as the benchmark for comparison. The inhibitory capacity of a compound was quantitatively represented through its IC50 value. The findings of this research revealed that the ethanol extract of Jamaica cherry exhibited an IC50 value of 158.262 ?g/mL, in contrast to acarbose, which demonstrated an IC50 of 0.256 ?g/mL. Keywords: Jamaica cherry (Muntingia calabura L.), alpha-glucosidase, IC50.
RESQINAH AZZAHRA. Aktivitas Inhibisi Enzim Alfa-Glukosidase Dari Ekstrak Bunga Kersen (Muntingia calabura L.) Secara In Vitro. (Dibimbing oleh St. Maryam dan Masdiana Tahir). Tumbuhan kersen merupakan salah satu tumbuhan yang sangat potensial untuk dimanfaatkan karena memiliki beberapa kandungan bioaktif yang bermanfaat untuk kesehatan salah satunya ialah flavonoid. Penelitian ini bertujuan untuk mengetahui aktivitas inhibisi enzim alfa-glukosidase dari ekstrak etanol bunga kersen (Muntingia calabura L.) secara in vitro menggunakan alat instrument Microplate reader. Metode penelitian ini diawali dengan penyiapan sampel dengan cara dimaserasi menggunakan pelarut etanol 96% lalu dilakukan uji penghambatan aktivitas enzim alfaglukosidase menggunakan substrat p-nitrofenil-?-D-glukopiranosida (PNPG) dan akarbosa sebagai pembanding. Potensi suatu senyawa dalam menghambat aktivitas enzim dapat diketahui melalui perhitungan IC50. Hasil penelitian menunjukkan bahwa ekstrak etanol bunga kersen (Muntingia calabura L.) memiliki nilai IC50 sebesar 158,262 ?g/mL dan nilai IC50 akarbosa sebesar 0,256 ?g/mL. Kata Kunci: Bunga kersen (Muntingia calabura L.), alfa-glukosidase, IC50 RESQINAH AZZAHRA. In Vitro Inhibition Assay of Alpha-Glucosidase of Jamaica Cherry Extract (Muntingia calabura L.). (Supervised by St. Maryam and Masdiana Tahir). The Muntingia calabura L., commonly referred to as Jamaica cherry, is recognized for its significant potential in therapeutic applications, primarily attributed to its rich bioactive constituents, notably flavonoids. The primary objective of this study was to elucidate the inhibitory activities of alphaglucosidase enzyme derived from the ethanol extract of Jamaica cherry using the microplate reader instrument. The experimental methodology entailed an initial phase of sample preparation, wherein Jamaica cherry underwent maceration with a 96% ethanol. Subsequent phases involved assessing the inhibitory potential of alpha-glucosidase, employing pnitrophenyl- ?-D-glucopyranoside (PNPG) as the substrate, with acarbose serving as the benchmark for comparison. The inhibitory capacity of a compound was quantitatively represented through its IC50 value. The findings of this research revealed that the ethanol extract of Jamaica cherry exhibited an IC50 value of 158.262 ?g/mL, in contrast to acarbose, which demonstrated an IC50 of 0.256 ?g/mL. Keywords: Jamaica cherry (Muntingia calabura L.), alpha-glucosidase, IC50.
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SAMPUL SKRIPSI RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
LEMBAR PENGESAHAN SKRIPSI RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
DAFTAR ISI SKRIPSI RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
ABSTRAK SKRIPSI RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
BAB 1 SKRIPSI RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
BAB V SKRIPSI RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
DAFTAR PUSTAKA RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
LAMPIRAN SKRIPSI RESQINAH AZZAHRA 15020190240 - Resqinah Azzahra.pdf
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