FUNGI ENDOFIT DAUN KECAPI (Sandoricum koetjape Merr.) SEBAGAI SENYAWA ANTIBAKTERI
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Institusion
Universitas Muslim Indonesia
Author
Ratnoto, Poppy Alfiani
Malik, Abd.
Yuliana, Dewi
Malik, Abd.
Yuliana, Dewi
Subject
L Education (General)
Datestamp
2024-10-10 03:30:13
Abstract :
Poppy Alfiani Ratnoto. Fungi Endofit Daun Kecapi (Sandoricum koetjape Merr.) Sebagai Senyawa Antibakteri. Dibimbing oleh bapak Rachmat Kosman dan bapak Herwin. Daun Kecapi (Sandoricum koetjape Merr) mengandung berbagai zat kimia seperti flavonoid, polifenol, saponin, dan terpenoid. Daun kecapi secara empiris dimanfaatkan sebagai obat diare, mulas, dan infeksi bakteri seperti bisul. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kecapi secara KLT-Bioautografi pada bakteri penyebab infeksi pada kulit. Hasil isolasi fungi endofit tumbuhan daun kecapi (Sandoricum koetjape Merr) memberikan aktivitas penghambatan pertumbuhan bakteri. Dari hasil pemurnian didapatkan isolate murni sebanyak 12 isolat dengan kode IFDK 01, IFDK 02, IFDK 03, IFDK 04, IFDK 05, IFDK 06, IFDK 07, IFDK 08, IFDK 09, IFDK 10, IFDK 11 dan IFDK 12. Hasil skrining aktivitas antibakteri dengan zona hambat terbesar pada isolat IFDK 05 menghambat bakteri Pseudomonas aeruginosa sebesar 16,334 mm dan isolat IFDK 12 menghambat bakteri Propionibacterium acne sebesar 10,334 mm. Hasil aktivitas antibakteri secara KLT-Bioautografi isolat berkode IFDK 05 dengan nilai Rf 0,91, 0,81, 0,58 menunjukkan bercak aktif pada bakteri Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, Pseudomonas aeruginosa dan nilai Rf 0,40 aktif terhadap bakteri Staphylococcus aureus dan Propionibacterium acne dan isolat IFDK 12 dengan nilai Rf 0,91 , 0,81 , 0,58 menunjukkan bercak aktif pada bakteri Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, Pseudomonas aeruginosa dan nilai Rf 0,40 aktif terhadap bakteri Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne dan Pseudomonas aeruginosa. Kata Kunci: Daun, Sandoricum koetjape Merr, KLT-Bioautografi, Antibakteri. ABSTRACT Poppy Alfiani Ratnoto. The antibacterial properties or compounds of Endophytic Fungi found in Lyre Leaves (Sandoricum koetjape Merr.) Under the supervision of Mr. Rachmat Kosman and Mr. Herwin. The leaves of Sandoricum koetjape Merr, commonly known as Kecapi leaves, are rich in a variety of chemical compounds including flavonoids, polyphenols, saponins, and terpenoids. Harp leaves have been observed to be efficacious in treating diarrhea, heartburn, and bacterial infections, including boils. The objective of this study is to assess the antibacterial effect of lyre leaf extract against bacteria responsible for skin infections using TLC-Bioautography. The isolation of the endophytic fungus from the harp leaf plant (Sandoricum koetjape Merr) yielded a substance that effectively inhibits bacterial growth. Based on the purification results, a total of 12 distinct isolates were obtained, each assigned a unique code: IFDK 01, IFDK 02, IFDK 03, IFDK 04, IFDK 05, IFDK 06, IFDK 07, IFDK 08, IFDK 09, IFDK 10, IFDK 11, and IFDK 12. Findings from activity assessment The antibacterial agent that exhibited the greatest zone of inhibition in isolate IFDK 05 effectively suppressed the growth of Pseudomonas aeruginosa bacteria by 16,334 mm, while isolate IFDK 12 inhibited the growth of Propionibacterium acne bacteria by 10,334 mm. The TLC-Bioautography analysis of the isolate coded IFDK 05 revealed active spots with Rf values of 0.91, 0.81, and 0.58, indicating antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, and Pseudomonas aeruginosa. Additionally, an Rf value of 0.40 exhibited antibacterial activity against unspecified bacteria. Staphylococcus aureus, Propionibacterium acne, and IFDK 12 isolates with Rf values of 0.91, 0.81, and 0.58 respectively exhibited active spots against Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, and Pseudomonas aeruginosa bacteria. Additionally, an Rf value of 0.40 showed activity specifically against Staphylococcus aureus bacteria, Staphylococcus epidermidis, Propionibacterium acne, and Pseudomonas aeruginosa. Keywords: Leaves, Sandoricum koetjape Merr, KLT-Bioautography, Antibacterial.
Poppy Alfiani Ratnoto. Fungi Endofit Daun Kecapi (Sandoricum koetjape Merr.) Sebagai Senyawa Antibakteri. Dibimbing oleh bapak Rachmat Kosman dan bapak Herwin. Daun Kecapi (Sandoricum koetjape Merr) mengandung berbagai zat kimia seperti flavonoid, polifenol, saponin, dan terpenoid. Daun kecapi secara empiris dimanfaatkan sebagai obat diare, mulas, dan infeksi bakteri seperti bisul. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kecapi secara KLT-Bioautografi pada bakteri penyebab infeksi pada kulit. Hasil isolasi fungi endofit tumbuhan daun kecapi (Sandoricum koetjape Merr) memberikan aktivitas penghambatan pertumbuhan bakteri. Dari hasil pemurnian didapatkan isolate murni sebanyak 12 isolat dengan kode IFDK 01, IFDK 02, IFDK 03, IFDK 04, IFDK 05, IFDK 06, IFDK 07, IFDK 08, IFDK 09, IFDK 10, IFDK 11 dan IFDK 12. Hasil skrining aktivitas antibakteri dengan zona hambat terbesar pada isolat IFDK 05 menghambat bakteri Pseudomonas aeruginosa sebesar 16,334 mm dan isolat IFDK 12 menghambat bakteri Propionibacterium acne sebesar 10,334 mm. Hasil aktivitas antibakteri secara KLT-Bioautografi isolat berkode IFDK 05 dengan nilai Rf 0,91, 0,81, 0,58 menunjukkan bercak aktif pada bakteri Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, Pseudomonas aeruginosa dan nilai Rf 0,40 aktif terhadap bakteri Staphylococcus aureus dan Propionibacterium acne dan isolat IFDK 12 dengan nilai Rf 0,91 , 0,81 , 0,58 menunjukkan bercak aktif pada bakteri Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, Pseudomonas aeruginosa dan nilai Rf 0,40 aktif terhadap bakteri Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne dan Pseudomonas aeruginosa. Kata Kunci: Daun, Sandoricum koetjape Merr, KLT-Bioautografi, Antibakteri. ABSTRACT Poppy Alfiani Ratnoto. The antibacterial properties or compounds of Endophytic Fungi found in Lyre Leaves (Sandoricum koetjape Merr.) Under the supervision of Mr. Rachmat Kosman and Mr. Herwin. The leaves of Sandoricum koetjape Merr, commonly known as Kecapi leaves, are rich in a variety of chemical compounds including flavonoids, polyphenols, saponins, and terpenoids. Harp leaves have been observed to be efficacious in treating diarrhea, heartburn, and bacterial infections, including boils. The objective of this study is to assess the antibacterial effect of lyre leaf extract against bacteria responsible for skin infections using TLC-Bioautography. The isolation of the endophytic fungus from the harp leaf plant (Sandoricum koetjape Merr) yielded a substance that effectively inhibits bacterial growth. Based on the purification results, a total of 12 distinct isolates were obtained, each assigned a unique code: IFDK 01, IFDK 02, IFDK 03, IFDK 04, IFDK 05, IFDK 06, IFDK 07, IFDK 08, IFDK 09, IFDK 10, IFDK 11, and IFDK 12. Findings from activity assessment The antibacterial agent that exhibited the greatest zone of inhibition in isolate IFDK 05 effectively suppressed the growth of Pseudomonas aeruginosa bacteria by 16,334 mm, while isolate IFDK 12 inhibited the growth of Propionibacterium acne bacteria by 10,334 mm. The TLC-Bioautography analysis of the isolate coded IFDK 05 revealed active spots with Rf values of 0.91, 0.81, and 0.58, indicating antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, and Pseudomonas aeruginosa. Additionally, an Rf value of 0.40 exhibited antibacterial activity against unspecified bacteria. Staphylococcus aureus, Propionibacterium acne, and IFDK 12 isolates with Rf values of 0.91, 0.81, and 0.58 respectively exhibited active spots against Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, and Pseudomonas aeruginosa bacteria. Additionally, an Rf value of 0.40 showed activity specifically against Staphylococcus aureus bacteria, Staphylococcus epidermidis, Propionibacterium acne, and Pseudomonas aeruginosa. Keywords: Leaves, Sandoricum koetjape Merr, KLT-Bioautography, Antibacterial.
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