Pengaruh Penggunaan Tali Keluh Untuk Handling Terhadap Kadar Hormon Kortisol Darah Sapi Peranakan Friesian Holstein
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Institusion
Universitas Airlangga
Author
Karinda Dylla Ayu Radhitama
Subject
Q Science
Datestamp
2020-12-30 13:34:02
Abstract :
The study was conducted to compare the response of stress between cattle through measuring serum cortisol hormone concentration which using a nose lead and without a nose lead. Handling aims to limit the space so that animals are easily controlled. Handling is done by attaching a nose lead with a stabbed into the septum nassale as the center of pain. Cortisol is a biomarker used to determine animal stress. The method of this research was complete random design. A total of twenty crossbreed Friesian Holstein were randomly divided into two groups, wich treated with halter rope (P1) and with nose lead (P2), then their blood were kept in vacutainer plain for measurement of the cortisol hormone concentration in the cattle serum by enzyme-linked immunosorbent assay (ELISA). The data were analysed with Shapiro-Wilk Test and continued with Independent T test. The result of the Independent T test is in each treatment was significantly different (p<0.05) with value p = 000 between treated without nose lead (28,147 ± 15,339) and with nose lead (63,147 ± 19,608). From the result, it can be concluded nose lead can increase cortisol serum activity.
The study was conducted to compare the response of stress between cattle through measuring serum cortisol hormone concentration which using a nose lead and without a nose lead. Handling aims to limit the space so that animals are easily controlled. Handling is done by attaching a nose lead with a stabbed into the septum nassale as the center of pain. Cortisol is a biomarker used to determine animal stress. The method of this research was complete random design. A total of twenty crossbreed Friesian Holstein were randomly divided into two groups, wich treated with halter rope (P1) and with nose lead (P2), then their blood were kept in vacutainer plain for measurement of the cortisol hormone concentration in the cattle serum by enzyme-linked immunosorbent assay (ELISA). The data were analysed with Shapiro-Wilk Test and continued with Independent T test. The result of the Independent T test is in each treatment was significantly different (p<0.05) with value p = 000 between treated without nose lead (28,147 ± 15,339) and with nose lead (63,147 ± 19,608). From the result, it can be concluded nose lead can increase cortisol serum activity.
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