Epitope Prediction Of B Cell Gene Encoding F Protein Of Newcastle Disease (Nd) Virus From Pigeon Isolate (Columbia Livia)
Total View This Week8
Total View This Week8
Institusion
Universitas Airlangga
Author
Yudha Kurniawan
Subject
SF780.2-780.7 Veterinary microbiology, bacteriology, virology, mycology
Datestamp
2021-06-29 08:04:40
Abstract :
Newcastle Disease is a highly contagious disease infecting avian animals caused by the ND virus that was previously synonymous with Avian Paramyxovirus type 1 (APMV-1). Even vaccination program management implemented, Indonesia is still an endemic area for ND. B cell epitope is a sequence of peptides (amino acids) found explicitly on specific antigens' surface, which binds to antibodies. Therefore, epitope characterization is very crucial for the development of vaccine making. There were 20 pigeon samples (Columba livia) collected from bird markets in East Java through the necropsy procedure. Three of them were positively infected by Newcastle Disease. Samples were collected in embryonated chicken eggs and identified by the HA test, confirmed by the HI test. PCR tests were performed on the positive samples using forward and reverse primers with a target of 329 bp. The sequencing result was then translated into an amino acid sequence via BioEdit software version 8.0. Prediction of immunogenic epitopes in genes protein F coding was performed using the online Epitope Prediction software Tools / IEDB with the Bepipred Linear Epitope Prediction. Epitope from sample ND / BG1 / 2019 more likely to be candidates for immunogenic epitopes based on predictions epitope B cell, which has the highest log score of 36.716.
Newcastle Disease is a highly contagious disease infecting avian animals caused by the ND virus that was previously synonymous with Avian Paramyxovirus type 1 (APMV-1). Even vaccination program management implemented, Indonesia is still an endemic area for ND. B cell epitope is a sequence of peptides (amino acids) found explicitly on specific antigens' surface, which binds to antibodies. Therefore, epitope characterization is very crucial for the development of vaccine making. There were 20 pigeon samples (Columba livia) collected from bird markets in East Java through the necropsy procedure. Three of them were positively infected by Newcastle Disease. Samples were collected in embryonated chicken eggs and identified by the HA test, confirmed by the HI test. PCR tests were performed on the positive samples using forward and reverse primers with a target of 329 bp. The sequencing result was then translated into an amino acid sequence via BioEdit software version 8.0. Prediction of immunogenic epitopes in genes protein F coding was performed using the online Epitope Prediction software Tools / IEDB with the Bepipred Linear Epitope Prediction. Epitope from sample ND / BG1 / 2019 more likely to be candidates for immunogenic epitopes based on predictions epitope B cell, which has the highest log score of 36.716.
File :
1. HALAMAN DEPAN.pdf
2. ABSTRACT.pdf
11. APPENDICES.pdf
KKC KK KH.97-21-embargo - Yudha Kurniawan.pdf
3. TABLE OF CONTENTS.pdf
4. CHAPTER I INTRODUCTION.pdf
5. CHAPTER II LITERATURE REVIEW.pdf
6. CHAPTER III MATERIALS AND METHODS.pdf
7. CHAPTER IV RESULT.pdf
8. CHAPTER V DISCUSSION.pdf
9. CHAPTER VI CONCLUSION AND SUGGESTION.pdf
10. REFERENCES.pdf
Download
book
BibTex
Latex, Jabref
cloud_download
Original Resource
url resource Institution
