Identifikasi Sequence Polimorfik Gen Promoter Follicle Stimulating Hormone-Receptor (Fshr) Pada Populasi Sapi Betina Friesian-Holstein Yang Mengalami Silent Heat
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Institusion

Universitas Airlangga

Author

Tantri Dyah Whidi Palupi

Subject

TS2284-2288 Animal feeds and feed mills. Pet food industry 

Datestamp

2021-08-22 03:21:49 

Abstract :
This study aims to determine the presence of changes in the arrangement of nucleotide bases found in the FSHR promoter gene sequence in silent heat cases in the field, to determine the difference between the FSHR promoter gene sequence which contained genetic variations in the silent-heat group and the gene sequence in the normal group, and to determine the effect of the presence of genetic variations in the promoter gene FSHR on cases of silent heat reproductive disorders in Friesian-Holstein cows. 10 samples of FH cows blood (5 SH, 5 Normal), DNA was extracted, then amplified. The amplification results were then examined by PCRRFLP, and followed by DNA sequencing. The results: there is a polymorphism in the form of single nucleotide polymorphism (SNP) in the SH group in the field, there is a difference between the FSHR promoter gene sequence that contains polymorphism in the SH group and the gene sequence in the normal group and there is no effect of polymorphism in the FSHR promoter gene on silent cases. heat in FH cows. 

File :
1. HALAMAN JUDUL.pdf
2. ABSTRACT.pdf
11. DAFTAR PUSTAKA.pdf
12. LAMPIRAN.pdf
PERMOHONAN EMBARGO.pdf
3. DAFTAR ISI.pdf
4. BAB I PENDAHULUAN.pdf
5. BAB II TINJAUAN PUSTAKA.pdf
6. BAB III KERANGKA KONSEPTUAL DAN HIPOTESIS.pdf
7. BAB IV MATERI DAN METODE PENELITIAN.pdf
8. BAB V ANALISIS HASIL PENELITIAN.pdf
9. BAB VI PEMBAHASAN.pdf
10. BAB VII KESIMPULAN DAN SARAN.pdf