PROFIL REKAM JEJAK DNA PADA LEMAK BABI MENTAH
DAN OLAHAN MENGGUNAKAN REAL-TIME POLYMERASE
CHAIN REACTION (REAL-TIME PCR)
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Institusion
Universitas Djuanda
Author
Zahra, Aulia
Subject
Q Science (General)
Datestamp
2022-03-28 08:37:22
Abstract :
ABSTRACT AULIA ZAHRA. B.1410646. Fingerprint of DNA from Raw and Processed Pork Fats Using Real-Time Polymerase Chain Reaction (Real-Time PCR). Supervised by Mardiah, Aminullah, and Raafqi Ranasasmita. Pork adulteration occurs frequently, and become a big problem for muslim. Real?time PCR is developed to detect a substance in molecular amount. The objective of the research was to determine the best DNA extraction method on fork fats, determine specific primers for amplification of DNA from pork fats and see the effect of the processing on the DNA produced. This research used three DNA extraction methods, namely Phenol Chloroform, Chelex 100, and Kogene Kit. The results showed that all three extraction methods could extract DNA from pork fats. The higest DNA yield was achieved by Phenol Chloroform method with an average concentration of 456.906 ng /µl; 2,022 A260/A280 ratio; Cq 15,1758; and Tm 86,274ºC. The specific primer for amplifying DNA from pork fats is D?LOOP22 primer because it has the lowest Cq value and has only one Tm value. The difference in extraction methods and processing has a real influence on the results of the concentration of DNA produced. This result shows that the research is capable of extracting DNA from pork fats. Keywords: Pork fats, DNA extraction method, Real-time PCR, Halal Verification.
ABSTRACT AULIA ZAHRA. B.1410646. Fingerprint of DNA from Raw and Processed Pork Fats Using Real-Time Polymerase Chain Reaction (Real-Time PCR). Supervised by Mardiah, Aminullah, and Raafqi Ranasasmita. Pork adulteration occurs frequently, and become a big problem for muslim. Real?time PCR is developed to detect a substance in molecular amount. The objective of the research was to determine the best DNA extraction method on fork fats, determine specific primers for amplification of DNA from pork fats and see the effect of the processing on the DNA produced. This research used three DNA extraction methods, namely Phenol Chloroform, Chelex 100, and Kogene Kit. The results showed that all three extraction methods could extract DNA from pork fats. The higest DNA yield was achieved by Phenol Chloroform method with an average concentration of 456.906 ng /µl; 2,022 A260/A280 ratio; Cq 15,1758; and Tm 86,274ºC. The specific primer for amplifying DNA from pork fats is D?LOOP22 primer because it has the lowest Cq value and has only one Tm value. The difference in extraction methods and processing has a real influence on the results of the concentration of DNA produced. This result shows that the research is capable of extracting DNA from pork fats. Keywords: Pork fats, DNA extraction method, Real-time PCR, Halal Verification.
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