Uji Aktivitas Ekstrak Etanol Daun Sirih Merah (Piper Crocatum) Terhadap Bakteri Propionibacterium Acnes
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Institusion
Universitas Kadiri
Author
Rahmaningtyas, Winda
Subject
RM Therapeutics. Pharmacology
Datestamp
2021-11-16 02:41:30
Abstract :
The purpose of this study is to identify the extract of red betel leaf ethanol (Piper crocatum) against Propionibacterium acnes bacteria. Red betel leaf was chosen because it is suspected to have active substances as antibacterial agents such as flavonoids, alkaloids, tannins, and essential oil. The study was conducted experimentally using a non-randomized control group design method with a prospective period. The extract was macerated using 96% ethanol solvent and evaporated until a thick extract was obtained. The extract was made in 3 different concentrations, namely 30%, 60%, and 90%. The positive control was clindamycin and the negative control was DMSO. The agar diffusion technique method was chosen to measure the diameter of the formed inhibition. The test results show that the average diameter of the inhibition zone formed by each sample is at a concentration of 30% of 7.40 mm, a concentration of 60% of 8.90 mm, and a concentration of 90% of 11.20 mm. Meanwhile, the results of data analysis using the Mann-Whitney method obtained a significant value of p <0.05, which indicates that there were significant differences between the experimental groups. The conclusion of this study is that the red betel leaf ethanol extract has activity against Propionibacterium acnes with the most effective concentration of 90%. Keywords: Red betel leaf (Piper crocatum), Ethanol Extract, Agar diffusion technique, Antibacterial, Propionibacterium acnes.
The purpose of this study is to identify the extract of red betel leaf ethanol (Piper crocatum) against Propionibacterium acnes bacteria. Red betel leaf was chosen because it is suspected to have active substances as antibacterial agents such as flavonoids, alkaloids, tannins, and essential oil. The study was conducted experimentally using a non-randomized control group design method with a prospective period. The extract was macerated using 96% ethanol solvent and evaporated until a thick extract was obtained. The extract was made in 3 different concentrations, namely 30%, 60%, and 90%. The positive control was clindamycin and the negative control was DMSO. The agar diffusion technique method was chosen to measure the diameter of the formed inhibition. The test results show that the average diameter of the inhibition zone formed by each sample is at a concentration of 30% of 7.40 mm, a concentration of 60% of 8.90 mm, and a concentration of 90% of 11.20 mm. Meanwhile, the results of data analysis using the Mann-Whitney method obtained a significant value of p <0.05, which indicates that there were significant differences between the experimental groups. The conclusion of this study is that the red betel leaf ethanol extract has activity against Propionibacterium acnes with the most effective concentration of 90%. Keywords: Red betel leaf (Piper crocatum), Ethanol Extract, Agar diffusion technique, Antibacterial, Propionibacterium acnes.
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