PENGARUH CAMPURAN NANOPARTIKEL TITANIUM DIOKSIDA PADA PLAT AKRILIK TERHADAP PERTUMBUHAN STREPTOCOCCUS SANGUINIS
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Institusion
Universitas Sriwijaya
Author
ROLISAH, YESA
Mozartha, Martha
Putri, Sekar
Mozartha, Martha
Putri, Sekar
Subject
RK1-715 Dentistry
Datestamp
2023-05-11 02:25:05
Abstract :
Background: Acrylic plates on removable orthodontic appliances can be a place for plaque and food scraps to facilitate Streptococcus sanguinis microorganisms to breed. Adding nanoparticle of titanium dioxide to acrylic plate may provide antibacterial activity. Objecttive: To see effect of TiO2 nanoparticle on acrylic plate to growth of Streptococcus sanguinis. Method: This research is a laboratory experimental research used acrylic plates without the addition of TiO2 nanoparticle and with the addition of TiO2 nanoparticle of 2%, 3%, and 4% with a size 10x10x1 mm. There were 24 samples grouped in 4 groups, each consisted of 6 samples sterilized with alcohol, then soaked on saline. The sample was put in Streptococcus sanguinis suspension, and than transferred to a tube containing PBS and then seeded on MSA media. The number of growing Streptococcus sanguinis colonies is calculated in units of CFU/ml. Data were analyzed statistically by Kruskal-wallis test and followed by Post-hoc test Mann Whitney. Result: There were significant differences in acrylic plates without addition of TiO2 nanoparticle and with the addition of TiO2 nanoparticle of 2%, 3%, and 4% to reduce the growth of Streptococcus sanguinis (P<0,05). The concentration of TiO2 nanoparticle 4% was the best to reduce the growth of Streptococcus sanguinis. Concusion: TiO2 nanoparticle addition acrylic plate have effect to reduce the growth of Streptococcus sanguinis. Key word: removable orthodontic appliance, TiO2 nanoparticle, acrylic plate, Streptococcus sanguinis.
Background: Acrylic plates on removable orthodontic appliances can be a place for plaque and food scraps to facilitate Streptococcus sanguinis microorganisms to breed. Adding nanoparticle of titanium dioxide to acrylic plate may provide antibacterial activity. Objecttive: To see effect of TiO2 nanoparticle on acrylic plate to growth of Streptococcus sanguinis. Method: This research is a laboratory experimental research used acrylic plates without the addition of TiO2 nanoparticle and with the addition of TiO2 nanoparticle of 2%, 3%, and 4% with a size 10x10x1 mm. There were 24 samples grouped in 4 groups, each consisted of 6 samples sterilized with alcohol, then soaked on saline. The sample was put in Streptococcus sanguinis suspension, and than transferred to a tube containing PBS and then seeded on MSA media. The number of growing Streptococcus sanguinis colonies is calculated in units of CFU/ml. Data were analyzed statistically by Kruskal-wallis test and followed by Post-hoc test Mann Whitney. Result: There were significant differences in acrylic plates without addition of TiO2 nanoparticle and with the addition of TiO2 nanoparticle of 2%, 3%, and 4% to reduce the growth of Streptococcus sanguinis (P<0,05). The concentration of TiO2 nanoparticle 4% was the best to reduce the growth of Streptococcus sanguinis. Concusion: TiO2 nanoparticle addition acrylic plate have effect to reduce the growth of Streptococcus sanguinis. Key word: removable orthodontic appliance, TiO2 nanoparticle, acrylic plate, Streptococcus sanguinis.
File :
RAMA_12201_04031281823035.pdf
RAMA_12201_04031281823035_TURNITIN.pdf
RAMA_12201_04031281823035_0005048108_01_front_ref.pdf
RAMA_12201_04031281823035_0005048108_02.pdf
RAMA_12201_04031281823035_0005048108_03.pdf
RAMA_12201_04031281823035_0005048108_04.pdf
RAMA_12201_04031281823035_0005048108_05.pdf
RAMA_12201_04031281823035_0005048108_06_ref.pdf
RAMA_12201_04031281823035_0005048108_07_lamp.pdf
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