INDUKSI KALUS EKSPLAN DAUN DUKU (Lansium domesticum Corr.) SECARA IN VITRO DENGAN PENAMBAHAN AUKSIN
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Institusion
Universitas Sriwijaya
Author
FARHAN FAHRUDDIN (STUDENT ID : 05091007054)
Susilawati Susilawati (LECTURER ID : 0008126701)
Astuti Kurnianingsih (LECTURER ID : 005066601)
Susilawati Susilawati (LECTURER ID : 0008126701)
Astuti Kurnianingsih (LECTURER ID : 005066601)
Subject
TJ1480-1496 Agricultural machinery. Farm machinery
Datestamp
2019-11-14 03:08:29
Abstract :
The aim of this research was to obtain the best callus formation in vitro culture by addition of 2,4-D (2,4-dicholorophenoxyacetic acid) and picloram (4-amino-3,5,6-tricloropicolinic acid) to induced duku?s leaf explants (Lansium domesticum Corr.). This research was conducted from February 2014 to October 2014 at Tissue Cultrue Laboratory, Department of Agronomy, Faculty of Agriculture, Sriwijaya University, Ogan Ilir, Palembang, South Sumatera. The culture medium which was used WPM (woody plant medium) with 2.4-D and picloram treatments and each of them contained of 0 ppm (control), 2 ppm, 4 ppm, and 8 ppm. This research was arranged in completly randomized design with 7 treatments and 5 replications. Each unit of treatment included of 6 culture bottles (sub replication) so that the total were 210 culture bottles. The result showed that duku?s leaf explant could make callus by additon of 2,4-D and picloram. Additin of 2,4-D with 4 ppm concentration had show the best result. The colour of growth callus was white, yellow to brown and also had the crumb texture.
The aim of this research was to obtain the best callus formation in vitro culture by addition of 2,4-D (2,4-dicholorophenoxyacetic acid) and picloram (4-amino-3,5,6-tricloropicolinic acid) to induced duku?s leaf explants (Lansium domesticum Corr.). This research was conducted from February 2014 to October 2014 at Tissue Cultrue Laboratory, Department of Agronomy, Faculty of Agriculture, Sriwijaya University, Ogan Ilir, Palembang, South Sumatera. The culture medium which was used WPM (woody plant medium) with 2.4-D and picloram treatments and each of them contained of 0 ppm (control), 2 ppm, 4 ppm, and 8 ppm. This research was arranged in completly randomized design with 7 treatments and 5 replications. Each unit of treatment included of 6 culture bottles (sub replication) so that the total were 210 culture bottles. The result showed that duku?s leaf explant could make callus by additon of 2,4-D and picloram. Additin of 2,4-D with 4 ppm concentration had show the best result. The colour of growth callus was white, yellow to brown and also had the crumb texture.
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RAMA_54211_05091007054_0008126701_005066601_01_front_ref.pdf
RAMA_54211_05091007054_0008126701_005066601_02.pdf
RAMA_54211_05091007054_0008126701_005066601_03.pdf
RAMA_54211_05091007054_0008126701_005066601_04.pdf
RAMA_54211_05091007054_0008126701_005066601_05.pdf
RAMA_54211_05091007054_0008126701_005066601_06_ref.pdf
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