Institusion
Sekolah Tinggi Ilmu Kesehatan Borneo Lestari
Author
Syahidaturrahmah, SYA
Ahmad Muhlisin, AM
Muhammad Arsyad (010912030), MA
Subject
D3 TLM, AAK Borneo Lestari
Datestamp
2019-08-14 02:27:57
Abstract :
QUALITY DIFFERENCE OF BLOOD SMEAR STAINING WITH WRIGHT'S
METHOD USED PDAM WATER , AQUADEST AND BUFFER PH 6,8.
Syahidatur Rahmah(1), Ahmad Muhlisin(2), Muhammad Arsyad(1)
Health Analyst Academy of Borneo Lestari Banjarbaru
Kelapa Sawit Street 8 Bumi Berkat Number 1
Phone. (0511) 5911626 Banjarbaru, South Borneo 70714
Email: syahidahrahmah@gmail.com
ABSTRACT
Blood smear is a thin smeared blood that is useful for looking at the
morphology of blood cells. there are various staining method for stain blood
smear ,one of which is wright staining. in wright staining , before blood smear
flooded with wright stain substance blood smear is flooded with buffer solution
first, recommended buffer acidity is 6,4 - 6,8. To determine quality difference of
blood smear staining with wright's method used pdam water , aquadest and
buffer ph 6,8. The research is an analytic survey, the sample used is venous
blood on one person as sample with 3 different treatments and 9 repetitions. On
the results of the above studies showed blood smear using aquadest obtained
with good quality blood smear as much as 100%, on blood smear using buffer pH
6,8 obtained with good quality blood smear as much as 63% and on blood smear
using PDAM water obtained with good quality blood smear as much as 54%.
From the results of statistical tests using non-parametric test kruskal willis test
method with statistical program for social sciences (SPSS) application. Acquired
asymp value. Sig of 0.002, Because the significant value obtained from the test
shows the value of 0.002 or <0.05 then H∘ rejected so that it can be concluded
that there are differences from 3 groups of examination. This study was
conducted without knowing exactly how the pH of aquades and PDAM water so
that it can be done again by measuring the pH of water in use before.
Keyword : blood smear, wright staining, aquadest, PDAM water, buffer Ph 6,8